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Your Position: Start > Antibody > CD71 > CD1-MY2101

Monoclonal Anti-CD71 Antibody, Mouse IgG1 (5B5) (MALS verified)

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  • Source

    Monoclonal Anti-CD71 Antibody, Mouse IgG1 (5B5) is a Mouse monoclonal antibody recombinantly expressed from HEK293 cells.

  • Clone

    CD71

  • Species

    Mouse

  • Isotype

    Mouse IgG1 | Mouse Kappa

  • Conjugate

    Unconjugated

  • Antibody Type

    Recombinant Monoclonal

  • Reactivity

    Human

  • Immunogen

    Recombinant Human CD71 is expressed from human 293 cells.

  • Specificity

    Specifically recognizes Human CD71.

  • Application
    ApplicationRecommended Usage
    ELISA0.1-63 ng/mL
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Purification

    Protein A purified/ Protein G purified

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • Cross Verification

    This product No cross-reactivity in ELISA with
    Rat Transferrin R, His Tag (Cat. No. TFR-R5249).
    Mouse Transferrin R, His Tag (Cat. No. TFR-M524b).

SDS-PAGE
CD71 SDS-PAGE

Monoclonal Anti-CD71 Antibody, Mouse IgG1 (5B5) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS
CD71 MALS images

The purity of Monoclonal Anti-CD71 Antibody, Mouse IgG1 (5B5) (Cat. No. CD1-MY2101) is more than 90% and the molecular weight of this protein is around 135-160 kDa verified by SEC-MALS.

Bioactivity-ELISA
 CD71 ELISA

Immobilized Cynomolgus Transferrin R, His Tag (Cat. No. TFR-C524a) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-CD71 Antibody, Mouse IgG1 (5B5) (Cat. No. CD1-MY2101) with a linear range of 0.1-8 ng/mL (QC tested).

 CD71 ELISA

Immobilized Human Transferrin R, His Tag (Cat. No. CD1-H5243) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-CD71 Antibody, Mouse IgG1 (5B5) (Cat. No. CD1-MY2101) with a linear range of 0.1-4 ng/mL (QC tested).

Bioactivity-SPR
 CD71 SPR

Captured Monoclonal Anti-CD71 Antibody, Mouse IgG1 (5B5) (Cat. No. CD1-MY2101) on CM5 chip via anti-mouse antibodies surface can bind Human Transferrin R, His Tag (Cat. No. CD1-H5243) with an affinity constant of 0.821 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).

  • Background
    CD antigen CD71 is also known as Transferrin receptor protein 1, TfR, sTfR, p90, TfR1, Trfr, which belongs to the peptidase M28 family and M28B subfamily. CD71 /TFR contains one PA (protease associated) domain. CD71 / TfR1 is required for iron delivery from transferrin to cells. CD71 is a potential new target in cases of human leukomia & lymphoma. CD71 /TFRC / TfR has been shown to interact with GABARAP and HFE.
  • Clinical and Translational Updates

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