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Ready-to-use Human iPSC-Derived Cerebral Organoids

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  • Product Details
    Human iPSC-Derived Cerebral Organoids are differentiated from hESC or iPSCs using Human iPSC-Derived Cerebral Organoid Differentiation Kit (Ca. No. RIPO-BWM001K). Cerebral organoids are three-dimensional in vitro models with a cellular composition and structural organization that is representative of the human cerebral regions. Organoids generated using Human iPSC-Derived Cerebral Organoid Differentiation Kit (Ca. No. RIPO-BWM001K) feature various types of neurons (including TH positive neurons) and glia cells (including OLIG2 and IBA1 positive cells). These cerebral organoids show spontaneous electrophysiological activity and response to a-syn PFFs induced toxicity, representing the functionality of the organoids.
  • Product Specification
    The live organoids are ready-to-use organoids that are delivered in shipping medium and has to go through a 48h recovery process according to instruction.
  • Storage

    After recovery, please store the organoid in its maintenance medium under the correct incubation condition and medium changing process.

Product Diagram
 - PRODUCT DIAGRAM

Protocol Diagram of cerebral organoid differentiation.

Validation Data
Orgnaoid Histology
 - ORGNAOID HISTOLOGY

Left: Early-stage cerebral organoid show rosette-like structures (neural stem cells), which become smaller as organoids develop.
Right: Day 109 cerebral organoids show uniform morphology and show no dead core inside.

Marker Expression
 - MARKER EXPRESSION

Presence of NESTIN positive cells and positive cells in early stage (day 36) cerebral organoid.
TUJ1 (beta III Tubulin), early stage neuronal marker
NESTIN : a cytoskeletal intermediate filament characterized in neural stem cells.

 - MARKER EXPRESSION

Presence of TH and MAP2 positive neurons in day 92 cerebral organoid.
TH: used as cell marker of dopaminergic neurons.
MAP2: mature neuron cell marker.

 - MARKER EXPRESSION

Left: Presence of GFAP positive cells at day 109 cultured cerebral organoid.
Right: Presence of OLIG2 positive cells at day 119 cultured cerebral organoid.
GFAP: marker for astrocyte.
OLIG2: marker for oligodendrocyte.

 - MARKER EXPRESSION

Presence of TH and IBA1 positive cell in day 147 cultured cerebral organoid.
IBA1: cell marker for microglia.

Organoid Activity
 - ORGANOID ACTIVITY

A: patch-clamp recording of excitatory neurons dissociated from cerebral organoids at day 102. Excitatory neuron show stable response to step injection currents.
B: Recording of cerebral organoid (day 60) using silicon probe. Neurons that show spontaneous activities have different waveforms.
C: MEA recording of cerebral organoid (day 86), spontaneous activities and bursting activities are recorded.

Organoid Application
 - ORGANOID APPLICATION

Using cerebral organoids for modelling Parkinson's Disease. Cerebral organoids are treated with Human Alpha-Synuclein Pre-formed Fibrils Protein (Ca. No. ALN-H51H4). Dopaminergic neurons and MAP2 positive neurons are damaged with both 0.1μM and 1μM Alpha-Synuclein Pre-formed Fibrils.

 - ORGANOID APPLICATION

AAVs selection using cerebral organoids: Different capsid of AAVs were incubated with cerebral organoids. As the results, different affinities of each AAV to the cerebral organoid were observed.

  • Clinical and Translational Updates

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Voraussichtlicher Liefertermin für vorrätige Produkte: 2 Arbeitstage

Price(EUR) : €160.00

Price(EUR) : €12930.00

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