Leave message
Can’t find what you’re looking for?
Fill out this form to inquire about our custom protein services!
Inquire about our Custom Services >>
Limited Edition Golden Llama is here! Check out how you can get one. 
Limited Edition Golden Llama is here! Check out how you can get one.
Offering SPR-BLI Services - Proteins provided for free!Get your ComboX free sample to test now!
Time Limited Offer: Welcome Gift for New Customers ! Shipping Price Reduction for EU Regions
| Assay Type | Inhibition-TR-FRET |
| Analyte | FcRn binding component |
| Format | 100T, 500T, 10000T |
| Reactivity | Human |
| Regulatory Status | RUO |
| Sensitivity | IC50=20.14μg/mL |
| Standard Curve Range | 0.24 μg/mL-1000 μg/mL |
| Assay Time | 1 hr |
| Suitable Sample Type | For the binding of IgG Fc region to the human FcRn receptor |
| Sample volume | 10 μL |
The Human FcRn Binding Kit (TR-FRET) takes advantage of binding of Europium-chelate labeled FcRn (donor) and FA labeled Human IgG1 antibody (acceptor) in a homogeneous (no wash) TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) competition assay to measure the interaction between human FcRn and antibody drug candidates or FcRn inhibitors. It is designed to facilitate the half-life evaluation of antibody drug candidates, and also high-throughput screening of FcRn inhibitors within 0.5-1 hours. It is highly sensitive, short detection time and easy to use.
This kit is designed to facilitate the half-life evaluation of antibody drug candidates, and also high-throughput screening of FcRn inhibitors. It can also be used as a universal detection tool to identifying the ability of antibody drugs to bind to FcRn.
It is for research use only.
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
| ID | Components | Size |
| FRT01-C01 | Human FCRN&B2M Heterodimer Protein Europium-chelate | 2.7 μg/100 tests 13.5 μg/500 tests 270 μg/10000 tests |
| FRT01-C02 | FA labeled human IgG antibody | 2.4 μg/100 tests 12 μg/500 tests 240 μg/10000 tests |
| FRT01-C03 | Human IgG standard | 200 μg/100 tests 1 mg/500 tests 20 mg/10000tests |
| FRT01-C04 | Sample Dilution Buffer | 10 mL/100tests & 500tests 100 mL/10000tests |
| FRT01-C05 | Detection Buffer | 10 mL/100tests & 500tests 100 mL/10000tests |
Inhibition of Europium-chelate labeled FcRn: FA labeled human IgG antibody binding by Human IgG
Premix serial dilutions of Human IgG standard (Catalog # FRT01-C03) (1:4 serial dilution, from 1000 μg/mL to 0.24 μg/mL (6666.67-1.63 nM)) and Human FCRN&B2M Heterodimer Protein Europium-chelate (Catalog # FRT01-C01) and incubate at room temperature (20℃-25℃) for 0.5 hour. Then add FA labeled human IgG antibody (Catalog # FRT01-C02) and incubate at room temperature (20℃-25℃) for 0.5 hour. Detection was performed with IC50 of 20.14 μg/mL. The assay was performed according to the above-described Datasheet (QC tested).
The half-lives of monoclonal antibodies currently in clinical use generally correlate with measurements of the affinity of their Fc for FcRn. The kit has been used to detect three FDA approved antibody drugs with different affinities binding to FcRn. The result is consistent with SPR test results. In the same experiment, the lower the IC50, the stronger binding affinity between IgG antibodies and FcRn, and the longer half-life of the antibody drug. Bevacizumab and Toripalimab have consistent binding affinity with FcRn and have a half-life of about 20 days. The Fc of Eculizumab has been modified to bind relatively weakly to FcRn, resulting in a relatively short half-life of approximately 11 days.
The kit has been used to detect the binding activity between Human FcRn and Human IgG standard (FRT-C03) under different pH. The affinity between Human FcRn and Human IgG standard (FRT-C03) decreases with increasing pH.
The kit has been used to detect different human IgG isoforms of Human IgG1, Human IgG2, Human IgG3, Human IgG4, with different affinities binding to FcRn.
The kit has been used to detect the Efgartigimod drug and Human IgG1 Fc (C103S, M135Y, S137T, T139E, H316K, N317F), His Tag (Cat.No. IG1-H52H8) that inhibit FcRn.
Price(EUR) : €760.00
Price(EUR) : €2530.00
Explore our catalog of therapeutic antibody solutions to find the right products for you! We are dedicated to delivering solutions designed to help you drive innovation and push the boundaries of what therapeutic antibodies can be.
We offer a wide range of cell and gene therapy solutions starting from discovery to the clinic. Explore our wide range of proteins, antibodies, kits, and other assays to accelerate the development of your cell and gene therapy.
Organoid Toolbox is a collection of organoid solutions including ready-to-use organoids, organoid differentiation kits, and a variety of services to accelerate the progress of your drug development project.
ACROBiosystems developed a series of GMP grade cytokines under the GMP grade quality management system. Those products are all suitable for T/NK cell generation, activation, and proliferation in cell therapy research.
50+ targets designed for CAR detection, including PE/FITC/biotin labeled proteins. The key reagents for CD19 and BCMA were FDA DMF filed which can support your IND, NDA and BLA process.
Full length multi-pass TPs with stabilized structure and high bioactivity for immunization, antibody screening, cell based assay and CAR detection, including hot CD20, Claudin 18.2， CD133, GPRC5D，CCR8, CCR5, etc.
GMP grade cytokines, reagents for cell activation, gene edition, DNA/RNA removal, etc. Particularly focus on product design, quality control and solution-based support to link each phase of your cell and gene therapy journey.
A series of immune checkpoints including classic co-inhibitory and co-stimulatory receptors. The comprehensive catalog contains 100+ targets with various species and tags, and the high-quality proteins are in good batch-to-batch consistency.
To meet the needs of ADCs development, ACROBiosystems can provide: A variety of high-quality target proteins; MMPs/Cathepsin/uPA for cleavable linker; Anti-payload antibodies & anti-idiotypic antibodies for immunogenicity and PK analysis; SPR/BLI analytical and ADA development service.
Comprehensive collection of Fc receptor proteins, including their common variants, which can help expedite your antibody development.
Comprehensive cytokine targets including interleukins, growth factors, chemokines, TNFs, etc. are expressed by HEK293 to ensure their natural structure. Their high purity is verified by SDS-PAGE/HPLC/SEC-MALS and high bioactivity is verified by ELISA/SPR/BLI.
Aneuro provides innovative solutions for neuroscience research. Recombinant proteins, neural factors, pre-formed fibrils, electrophysiological electrodes, as well as Organoid Toolbox all in Aneuro aiming to advance neuroscience research, develop therapeutic interventions, and improve diagnostic methods for neurological diseases.
This web search service is supported by Google Inc.
A-Z
