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IgG-Isotypkontrollen

IgG-Isotypkontrollen: IgG-Isotypkontrollen
IgG-Isotypkontrollen

Antikörper-Medikamente sind zu einem wichtigen Bereich in der Entwicklung der Biomedizin geworden. Isotypkontroll-Antikörper in der Entwicklung von Antikörper-Medikamenten gehören zur gleichen Klasse und zum gleichen Subtyp wie der gegen das Zielmolekül entwickelte primäre Antikörper, binden aber nicht spezifisch an ein bekanntes oder potenzielles Zielmolekül. Mit der Isotypkontrolle soll bestätigt werden, dass die Bindung oder Wirkung des primären Antikörpers spezifisch ist und unspezifische Wechselwirkungen mit Fc-Rezeptorproteinen oder mit anderen Proteinen ausgeschlossen sind. Inzwischen kann die Isotypkontrolle auch als Antikörper verwendet werden, der um die Bindung an Fc-Rezeptorproteine konkurriert, was mit funktionellen blockierenden Antikörpern verwandt ist, die die gleiche Funktion erfüllen, was eine effektive und angemessene Bewertung der Wirksamkeit von Antikörper-Medikamenten erleichtert. Der unspezifische Hintergrund ist eine wichtige Variable, die kontrolliert werden muss. Die Einstellung des entsprechenden Isotypkontroll-Antikörpers zum Nachweis unspezifischer Bindungen kann die Entstehung falsch positiver Ergebnisse verringern und die möglichen Einflussfaktoren im Prozess der Arzneimittelentwicklung genau bewerten.

ACROBiosystems hat eine Reihe von IgG Isotype-Kontrollen für Mensch- und Mausspezies mit verschiedenen Subtypen entwickelt, die als Isotypkontroll-Antikörper in verschiedenen Anwendungen wie ELISA, western blot, Immunofluoreszenz, Immunohistochemie (IHC) und Durchflusszytometrie (FACS) usw. verwendet werden können.

IgG-Isotypkontrollen Produktliste
MoleculeCat. No.SpeciesProduct DescriptionPreorder/Order
IgG ControlDNP-M2HumanHuman IgG1 Kappa Isotype Control (mAb, carrier free, MALS verified)

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DNP-BM2Biotinylated Human IgG1 Kappa Isotype Control (Biotin, mAb, carrier free, MALS verified)

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DNP-MB273Human IgG1 (N297A) Kappa Isotype Control (mAb, carrier free, MALS verified)

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DNP-M915Human IgG3 Kappa Isotype Control (mAb, carrier free)

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DNP-M3Human IgG4 Kappa Isotype Control (mAb, carrier free, MALS verified)

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DNP-FM2FITC-Labeled Human IgG1 Kappa Isotype Control (FITC, mAb, carrier free)

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DNP-M1MouseMouse IgG1 Kappa Isotype Control (mAb, Carrier free, MALS verified)

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DNP-BM1Biotinylated Mouse IgG1 Isotype Control (Biotin, mAb, carrier free, MALS verified)

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DNP-M1A1Mouse IgG1 kappa Isotype Control

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DNP-BM190Biotinylated Mouse IgG1 kappa Isotype Control

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DNP-FM1FITC-Labeled Mouse IgG1 Isotype Control (FITC, mAb, carrier free)

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DNP-FM1A1FITC-Labeled Mouse IgG1 Isotype Control

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DNP-PM1PE-Labeled Monoclonal Mouse IgG1 Isotype Control (Site-specific conjugation)

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IgG-Isotypkontrollen Überprüfungsdaten

Hohe Reinheit und strukturelle Homogenität bestätigt durch SDS-PAGE und SEC-MALS

purity_ACA-H421b

Human IgG1 (N297A) Kappa Isotype Control (mAb) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%. The purity of Human IgG1 (N297A) Kappa Isotype Control (mAb) (Cat. No. DNP-MB273) is more than 90% and the molecular weight of this protein is around 140-160 kDa verified by SEC-MALS.

purity_GCF-H5214

Mouse IgG1 Kappa Isotype Control (mAb) on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%. The purity of Mouse IgG1 Kappa Isotype Control (mAb) (Cat. No. DNP-M1) is more than 90% and the molecular weight of this protein is around 130-160 kDa verified by SEC-MALS.

Hohe biologische Aktivität, bestätigt durch ELISA

DNP-M2-E1

Immobilized DNP-Albumin Conjugate at 10 μg/mL (100 μL/well) can bind Human IgG1 Kappa Isotype Control (mAb) (Cat. No. DNP-M2) with a linear range of 1-10 ng/mL (QC tested).

DNP-M3-E1

Immobilized DNP-Albumin Conjugate at 10 μg/mL (100 μL/well) can bind Human IgG4 Kappa Isotype Control (mAb) (Cat. No. DNP-M3) with a linear range of 1-15 ng/mL (QC tested).

DNP-MB273-E1

Immobilized DNP-Albumin Conjugate at 10 μg/mL (100 μL/well) can bind Human IgG1 (N297A) Kappa Isotype Control (mAb) (Cat. No. DNP-MB273) with a linear range of 1-10 ng/mL (QC tested).

DNP-M1-E1

Immobilized DNP-Albumin Conjugate, Bovine at 10 μg/mL (100 μL/well) can bind Mouse IgG1 Kappa Isotype Control (mAb) (Cat. No. DNP-M1) with a linear range of 2-20 ng/mL (QC tested).

FACS-bestätigt für die Verwendung als Isotypkontrollen

DNP-BM1-F3

2e5 of Anti-CD19 CAR-293 cells were stained with 100 μL of 1 μg/mL of Biotinylated Mouse IgG1 Isotype Control (mAb) (Cat. No. DNP-BM1) and positive control respectively, washed and then followed by PE-SA and analyzed with FACS (Routinely tested).

DNP-FM1-F3

2e5 of Anti-CD19 CAR-293 cells were stained with 100 μL of 1 μg/mL of FITC-Labeled Mouse IgG1 Isotype Control (mAb) (Cat. No. DNP-FM1) and positive control respectively, and FITC signal was used to evaluate the binding activity (Routinely tested).

DNP-M1A1-F1

2e5 of anti-CD19 CAR-293 cells were stained with 100ul of 1:50 dilution of 2e5 of anti-CD19 CAR-293 cells were stained with 100ul of 1:50 dilution of Mouse IgG1 kappa Isotype Control (Cat. No. DNP-M1A1) and positive control antibody respectively, washed and then followed by PE anti-mouse IgG1 and analyzed with FACS (QC tested).

DNP-BM190-F1

2e5 of anti-CD19 CAR-293 cells were stained with 100 μL of 1:50 dilution (2 μL stock solution in 100 μL FACS buffer) Biotinylated Mouse IgG1 kappa Isotype Control (Cat. No. DNP-BM190) and positive control antibody (Cat. No. FM3-BY54) respectively, washed and then followed by PE-SA and analyzed with FACS (QC tested).

DNP-FM1A1-F1

2e5 of Anti-CD19 CAR-293 cells were stained with 100 μL of 1:50 dilution (2 μL stock solution in 100 μL FACS buffer) of FITC-Labeled Mouse IgG1 Isotype Control (Cat. No. DNP-FM1A1) and positive control (Cat. No. FM3-FY45P1) respectively, and FITC signal was used to evaluate the binding activity (QC tested).

DNP-PM1-F1

5e5 of Anti-CD19 CAR-293 cells were stained with 100 μL of 1:50 dilution (2 μL stock solution in 100 μL FACS buffer) of PE-Labeled Monoclonal Mouse IgG1 Isotype Control (Cat. No. DNP-PM1) and positive control (Cat. No. FM3-HPY53) respectively, and PE signal was used to evaluate the binding activity (QC tested).

Andere Isotypkontrollen

IgG Fc Proteins——Ideal Isotype Control for IgG Structured Drug Development
ACRO Quality

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