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Human iPSC-Derived Intestinal Organoid Differentiation Kit

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Box A(2-8°C)
IDComponentsSize
RIPO-IWM005K-C01Intestinal organoid Basal Medium B13ml
RIPO-IWM005K-C02Intestinal organoid Basal Meidium C13ml
RIPO-IWM005K-C03Intestinal organoid Basal Medium D100ml
  • Product Details
    Human iPSC-Derived Intestine Organoid Differentiation Kit (Ca. No. RIPO-IWM005K) allows hESC or hiPSC to differentiate into intestine organoids. Intestine organoids are three-dimensional in vitro models with a cellular composition and structural organization that is representative to the human intestine regions. This kit can produce 48 intestinal organoids in four steps. Organoids generated using Human iPSC-Derived intestine Organoid Differentiation Kit (Ca. No. RIPO-IWM005K) feature various types of cells, including intestine epithelium cells, mesenchyme cells, enterocytes, Paneth cells, goblet cells, etc. These intestine organoids show intestine crypt like structure, Villi and Microvilli like structure. These intestine organoids show normal intestinal function validated by the absorption of fatty acid and glucose.
  • Product Specification
    The basic medium of this differentiation kit is a serum-free, well-defined medium with minimal batch variation to which differentiation factors are added. This medium does not contain antibiotics, the addition of which may affect organoid differentiation.
  • Storage

    The unopened Box A is stable for 12 months from the date of manufacture if stored under 2-8°C.
    The unopened Box B is stable for 12 months from the date of manufacture if stored under -20°C.
    The opened kit should be stored according to components table.

Product Diagram
 - PRODUCT DIAGRAM

Protocol Diagram of intestine organoid differentiation.

Validation Data
Organoid Histology and State
 - Organoid Histology And State

The intestine organoids differentiated using the Human iPSC-Derived intestine Organoid Differentiation Kit (Ca. No. RIPO-IWM005K) show regular peristalsis.

Orgnaoid Histology
 - ORGNAOID HISTOLOGY

Observation of granulocyte like cells, adipocyte like cells, smooth muscle like cells and intestinal crypt like structure by morphology on day 120 intestine organoids.

Marker Expression
 - MARKER EXPRESSION

The intestine organoids differentiated using the Human iPSC-Derived intestine Organoid Differentiation Kit (Ca. No. RIPO-IWM005K) show expression of goblet cells (mucus-producing, MUC2), brush borders (Villin) and endothelial cells (CD31).

 - MARKER EXPRESSION

The intestine organoids differentiated using the Human iPSC-Derived intestine Organoid Differentiation Kit (Ca. No. RIPO-IWM005K) show expression of enterochromaffin cells (CHGA) and endothelial cells (CD31).

 - MARKER EXPRESSION

The intestine organoids differentiated using the Human iPSC-Derived intestine Organoid Differentiation Kit (Ca. No. RIPO-IWM005K) show expression of goblet cells (mucus-producing, MUC2) and endothelial cells (CD31).

 - MARKER EXPRESSION

The intestine organoids differentiated using the Human iPSC-Derived intestine Organoid Differentiation Kit (Ca. No. RIPO-IWM005K) show expression of smooth muscle cell (α-SMA); epithelial cell (EPCAM); macrophage (CD68); goblet cell (MUC2); Ki67 (intestinal stem cell) and enteroendocrine cells (CHGA).

Organoid Activity
 - ORGANOID ACTIVITY

Intestine organoids differentiated using the Human iPSC-Derived intestine Organoid Differentiation Kit (Ca. No. RIPO-IWM005K) show normal intestinal function validated by the absorption of fatty acid and glucose.

  • Clinical and Translational Updates

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