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> Enzyme-Linked Immunosorbent Assay (ELISA)
Enzyme Linked Immunosorbent Assays (ELISA) sind seit Jahrzehnten eine der wichtigsten Methoden zum Nachweis von Analyten. Als Zugpferd unter den Experimenten zu jeglicher Arzneimittelentwicklungsforschung sind hochwertige und zuverlässige ELISA-Daten für das wissenschaftliche Verständnis und weitere Untersuchungen von entscheidender Bedeutung.
Der Vorteil von ACRO resultiert aus unseren qualifizierten und erfahrenen Wissenschaftlern, dem großen Katalog an selbst hergestellten Proteinen und Antikörpern, einschließlich Anti-Idiotyp-Antikörpern, sowie unseren gebrauchsfertigen ELISA-Kits.
Wir bieten einen umfassenden Service für die Entwicklung von ELISA-Bioassay-Methoden, einschließlich Methodenentwicklung, Validierung, Transfer und Kit-Entwicklung entsprechend Ihren Anforderungen, damit Sie Ihren Arzneimittelentwicklungsprozess beschleunigen können!
ACROBiosystems führt hochwertige Absorptions-, Fluoreszenz- und Lumineszenzmessungen mit dem SpectraMax i3x System (Molecular Devices) durch. ACROBiosystems hat auch die ScanLaterTM Western Blot-Erweiterung sowie die Alpha Screen 384 STD-Detektionskartusche erworben, um unseren Kunden mehrere Analyseoptionen zu bieten.
Empfindlichkeit über das gesamte Spektrum mit Spectral Fusion™-Illumination
Erweiterter Dynamikbereich mit gekühltem PMT
Kartuschen für FP, AlphaScreen und Western Imaging
Basierend auf dem SpectraMax i3x System bietet ACROBiosystems biomedizinische Forschungs- und Entwicklungsdienstleistungen für Kunden an, die eine Quantifizierung und Detektion von Antikörper-Antigen-Interaktionen wünschen. Weitere Dienstleistungen umfassen unter anderem Anti-Drogen-Aktivität (ADA), PK/PD-Tests und Antikörper-Screenings.
Der Analysebericht wird innerhalb von 2 Wochen nach Erhalt der Probe zugestellt!
ACROBiosystems bietet ein umfassendes Angebot an Dienstleistungen für die Entwicklung von ELISA-Bioassay-Methoden, angefangen bei den Testreagenzien bis hin zum Probennachweis!
Entwicklung von ELISA-Bioassay-Methoden aus einer Hand
We provide screening services including peptide screening and antibody screening using common methods like competitive ELISA.
For example, we can perform a screening assay for peptide samples using ACROBiosystem's own Anti-SARS-CoV-2 Neutralizing Antibody Titer Serologic Kit (Cat#: RAS-N022).
The microplate in the kit has been pre-coated with Human ACE2 protein. First serum samples, Positive control, Negative Control are added to the wells followed by addition of HRP-SARS-CoV-2 Spike RBD. The presence of neutralizing antibodies in samples will compete with ACE2 for HRP-SARS-CoV-2 Spike RBD binding. The intensity of assay signal decrease proportionally to the presence of Anti-SARS-CoV-2 neutralizing antibody.
Fig1. Positive reference for anti-SARS-CoV-2 neutralizing antibody concentrations (μg/mL) for peptide 9 screening (SpectraMax i3x).
Fig2. Sample screening for peptide 9 (uM) using anti-SARS-CoV-2 neutralizing antibody titer serologic Kit (Cat#: RAS-N022) (SpectraMax i3x).
Fig3. Different anti-BCMA antibodies have different neutralization ability for BCMA and BAFF.
ln addition to antigen affinity screening, affinity of antibody to Fc receptors and C1q is responsible for cell killing via ADCC/ADCP and CDC effects respectively. Therefore testing association of Fc receptors and C1q is necessary during the development phase of antibody drug candidates.
Fig4. Standard curve of mouse complement C3a to determine the concentrations of C3a in client mouse plasma samples (SpectraMax i3X).
We can perform a binding assay for Human TROP-2 and a client’s antibody to test the efficacy of the provided antibody using our own Human TROP-2 / TACSTD2 Protein, His Tag (Cat#: TR2-H5223). The microplate is coated overnight with TROP-2. The sample antibody is added to the wells, and subsequently the secondary HRP conjugated Goat Anti-Mouse IgG is added. After the primary antibody from the client binds to TROP-2 the HRP secondary antibody will bind to the primary, and following the addition of TMB the intensity of the signal will be proportional to the concentration of primary antibody.
Fig5. Binding curve comparing the concentration of anti-TROP-2 antibody (nM) to the optical density to determine the EC50 (SpectraMax i3x).
An idiotope is the unique set of antigenic determinants (epitopes) of the variable portion of an antibody. An anti-idiotypic (Anti-ID) antibody binds to the idiotope of another antibody, usually an antibody drug, which makes it a very powerful tool for antibody drug development, especially for immunogenicity and PK/PD analysis.
To support preclinical/clinical immunogenicity and PK analysis, ACROBiosystems has developed a series of high-affinity anti-idiotypic antibodies and ADA/PK services.
Fig6. Immobilized Adali**mab at 1 µg/ml, add increasing concentrations of anti-Adali**mab antibodies (Cat. No. ADB-Y19, 10% human serum) and then add biotinylated Adali**mab at 5 µg/ml. Detection was performed using HRP-conjugated
ACRO has developed conjugated magnetic beads which can be used in beads based ELISA.
ACROBiosystems scientist will respond within 24 hours of submission.
Call us at: +1 800-810-0816 or email at services@acrobiosystems.com for consultation.
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