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Anti-CD19 (FMC63) CAR Immunogenicity ELISA Kit

For research use only.

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RAB001-C01High-bind Plate1 plate
RAB001-C02Mouse FMC63 scFv25 μg
RAB001-C03FMC63 ADA Standard15 μg
RAB001-C04Biotin-Mouse FMC63 scFv20 μg
RAB001-C05Streptavidin-HRP50 μL
RAB001-C06Coating Buffer12 mL
RAB001-C0710xWashing Buffer 50 mL
RAB001-C08Blocking Buffer50 mL
RAB001-C09Substrate Solution12 mL
RAB001-C10Stop Solution7 mL
  • Background
    FMC63 is an IgG2a mouse monoclonal antibody specific for CD19, which is a target for the immunotherapy of B lineage leukaemias and lymphomas. FMC63 scFv is the most commonly used ectodomain component of CD19-specific CARs. So far, most of reported CART19 trials contain the anti-CD19 scFv derived from FMC63, including the two FDA-approved CARs Kymriah and Yescarta.Anti-fmc63 scFv antibody can specifically bind to the antigen recognition epitope of fmc63 scFv on anti-CD19 car, and it shows high specificity and sensitivity,it's used to detect the expression of fmc63 scFv derived car.
  • Application

    This kit is developed for detection of Anti-CD19 (FMC63) antibodies in CAR Immunogenicity assay test. Detection and monitoring the level of ADA is important to fully understand the potential immune responses.

    It is for research use only.

  • Reconstitution
    Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
  • Storage & Shipping
    The unopened kit is stable for 12 months from the date of manufacture if stored at 2°C to 8°C.

    The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

    The kit shipped at room temperature that had been validated. Please contact us if you need blue ice shipping, but additional freight may be followed.

    Below is the Shipping Statement for Kit Products.

    Shipping Statement

  • Assay Principles
    This assay kit employs a bridging-ELISA format, providing a rapid detection of FMC63 ADA. The kit consists High-bind Plate and Mouse FMC63 scFv and FMC63 ADA Standard and Biotin-Mouse FMC63 scFv and Streptavidin-HRP and buffers.

    Your experiment will include 4 simple steps:

    a) Coat the plate with Mouse FMC63 scFv.

    b) Add your molecule of interest to the tests.

    c) Add Mouse FMC63 scFv-Biotin.

    d) Add Streptavidin-HRP followed by TMB or other colorimetric HRP substrate.

Typical Data Please refer to Ds document for the assay protocol.

Detection of FMC63 ADA titer by bridging-ELISA Assay.
Immobilized Mouse FMC63 scFv can bind FMC63 ADA in 1:20 human serum, and then add Biotin-Mouse FMC63 scFv. Detection was performed using HRP-conjugated streptavidin with sensitivity of 19 ng/mL (QC tested).

  • Clinical and Translational Updates

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Price(EUR) : €1210.00

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