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Overexpression cell line

Overexpression cell line
Background
Overexpression stable cell lines are a genetically modified stable cell line designed to continuously overexpress a specific gene. A specific gene is designed to increase the expression level and subsequently introduced into cells using methods such as lentiviruses and electroporation to achieve ‘Gain-of-Function’. The resulting cell then expresses the target antigen on the cell membrane surface, a function which is retained across multiple cell passages. This is a traditional molecular biology technique to study gene function which still plays a significant role in biological research.
Based on the mature cell construction platform, ACROBiosystems continues to develop high-quality overexpression cell line products, providing stable cell lines for gene research and development tool for new drug development!

Application scenarios

  1. >Gene Function Research

  2. >Immunity/killing/proliferation, etc.

  3. >Activity screening of antibody drugs (binding and blocking at the cellular level)

  4. >Cytotoxicity evaluation of CAR therapies

Verification data
Receptor Assay
Receptor Assay

Expression analysis of human CD64 on CHO/Human CD64 Stable Cell Line by FACS. Cell surface staining using PE-labeled anti-human CD64 antibody was performed on CHO/Human CD64 Stable Cell Line with different expression levels: CHO/Human CD64 Stable Cell Line (Low Expression) (Cat. No. SCCHO-ATP062L); CHO/Human CD64 Stable Cell Line (Medium Expression) (Cat. No. SCCHO-ATP062M); CHO/Human CD64 Stable Cell Line (High Expression) (Cat. No. SCCHO-ATP062H).

Application
Application

Bioactivity analysis of anti-human 4-1BB antibody through CHO/Human CD32b Stable Cell Line (Medium Expression) (Cat. No. SCCHO-ATP060M) crosslinking to test whether in a CD32b-dependent manner to strengthen the agonistic activity. The EC50 of anti-human 4-1BB antibody is approximately 0.18 μg/mL through CHO/Human CD32b Stable Cell Line (Medium Expression) crosslinking.

Application

Blocking activity of anti-human PD-1 antibody (FOLD). This Raji/Human PD-L1 Stable Cell Line (Cat. No. SCRAJ-STT075) was incubated with serial dilutions of antibodies in the presence of reporter cells expressing human PD-1. The max induction fold was approximately 22.47.

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