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Biotinylated Human / Cynomolgus / Rhesus macaque ROR1 Protein, Avitag™ (MALS verified)

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  • Synonym
    ROR1,NTRKR1
  • Source
    Biotinylated Human / Cynomolgus / Rhesus macaque ROR1, Avitag (RO1-H821y) is expressed from human 293 cells (HEK293). It contains AA Gln 30 - Glu 403 (Accession # Q01973-1). In the region Gln 30 - Glu 403, the AA sequence of Human, Cynomolgus and Rhesus macaque ROR1 are homologus.
    Predicted N-terminus: Gln 30
  • Molecular Characterization
    ROR1 Structure

    This protein carries an Avi tag (Avitag™) at the C-terminus.

    The protein has a calculated MW of 45.2 kDa. The protein migrates as 55-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
ROR1 SDS-PAGE

Biotinylated Human / Cynomolgus / Rhesus macaque ROR1, Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
ROR1 MALS images

The purity of Biotinylated Human / Cynomolgus / Rhesus macaque ROR1, Avitag (Cat. No. RO1-H821y) is more than 90% and the molecular weight of this protein is around 50-70 kDa verified by SEC-MALS.

Bioactivity-ELISA
 ROR1 ELISA

Immobilized Anti-ROR1 Antibody, Human IgG1 at 1 μg/mL (100 μL/well) can bind Biotinylated Human / Cynomolgus / Rhesus macaque ROR1, Avitag (Cat. No. RO1-H821y) with a linear range of 0.4-13 ng/mL (QC tested).

Bioactivity-FACS
 ROR1 FACS

2e5 of anti-ROR1 CAR-293 cells were stained with 100 μL of 3 μg/mL of Biotinylated Human / Cynomolgus / Rhesus macaque ROR1, Avitag (Cat. No. RO1-H821y) and negative control protein respectively, washed and then followed by PE-SA and analyzed with FACS (Routinely tested).

  • Background
    Tyrosine-protein kinase transmembrane receptor ROR1 is also known as Neurotrophic tyrosine kinase, receptor-related 1 (NTRKR1), which belongs to the protein kinase superfamily or tyr protein kinase family or ROR subfamily. ROR1 contains 1 FZ (frizzled) domain, 1 Ig-like C2-type (immunoglobulin-like) domain, 1 kringle domain, 1 protein kinase domain. ROR1 is expressed at high levels during early embryonic development. The expression levels drop strongly around day 16 and there are only very low levels in adult tissues. Isoform Short is strongly expressed in fetal and adult CNS and in a variety of human cancers, including those originating from CNS or PNS neuroectoderm. ROR1 could interact with casein kinase 1 epsilon (CK1ε) to activate phosphoinositide 3-kinase-mediated AKT phosphorylation and cAMP-response-element-binding protein (CREB), which was associated with enhanced tumor-cell growth.
  • Clinical and Translational Updates

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