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Your Position: Start > Protein > Envelope protein H&L&UL128&UL130&UL131 > ENN-V5283

Human cytomegalovirus (strain Merlin) (HHV-5) Envelope protein H&L&UL128&UL130&UL131, Twin-Strep Tag&His Tag (MALS verified)

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  • Source
    Human cytomegalovirus Envelope protein H&L&UL128&UL130&UL131, Twin-Strep Tag&His Tag(ENN-V5283) is expressed from human 293 cells (HEK293). It contains AA Ala 31 - Arg 278 & Arg 24 - Leu 719 (H&L) & Glu 28 - Gln 171 (UL128) & Ser 26 - Val 214 (UL130) & Gln 19 - Asn 129 (UL131) (Accession # Q6SW67 & F5HCH8 (H&L) & V9LLX6 (UL128) & F5HCP3 (UL130) & F5HET4 (UL131)).
    Predicted N-terminus: Trp (H&L) & Glu 28 (UL128) & Ser 26 (UL130) & Gln 19 (UL131)
  • Molecular Characterization

    The protein has a calculated MW of 110.8 kDa (H&L) & 16.7 kDa (UL128) & 23.7 kDa (UL130) & 13.0 kDa (UL131). The protein migrates as 15 kDa,35 kDa and 130 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Envelope protein H&L&UL128&UL130&UL131 SDS-PAGE

Human cytomegalovirus Envelope protein H&L&UL128&UL130&UL131, Twin-Strep Tag&His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS
Envelope protein H&L&UL128&UL130&UL131 MALS images

The purity of Human cytomegalovirus Envelope protein H&L&UL128&UL130&UL131, Twin-Strep Tag&His Tag (Cat. No. ENN-V5283) is more than 85% and the molecular weight of this protein is around 170-200 kDa verified by SEC-MALS.

Bioactivity-ELISA
 Envelope protein H&L&UL128&UL130&UL131 ELISA

Immobilized Human Neuropilin-2, His Tag (Cat. No. NR2-H52H3) at 5 μg/mL (100 μL/well) can bind Human cytomegalovirus Envelope protein H&L&UL128&UL130&UL131, Twin-Strep Tag&His Tag (Cat. No. ENN-V5283) with a linear range of 0.004-0.125 μg/mL (QC tested).

Bioactivity-SPR
 Envelope protein H&L&UL128&UL130&UL131 SPR

Human Neuropilin-2, His Tag (Cat. No. NR2-H52H3) immobilized on CM5 Chip can bind Human cytomegalovirus Envelope protein H&L&UL128&UL130&UL131, Twin-Strep Tag&His Tag (Cat. No. ENN-V5283) with an affinity constant of 3.41 µM as determined in a SPR assay (Biacore 8K) (Routinely tested).

  • Background
    Human cytomegalovirus (HCMV), a prototypical beta-herpes virus, is a major cause of morbidity and mortality in immunocompromised transplant recipients and congenitally infected fetuses.Different HCMV glycoprotein complexes are capable of meditating entry through interaction with cell-specific receptors on a panel of host cells. The pentamer (gH/gL/UL128/UL130/UL131A) mediates HCMV entry into epithelial, endothelial, and myeloid cells by its binding to neuropilin 2 (Nrp2). The trimer (gH/gL/gO) can mediate infection of all cell types by binding to platelet-derived growth factor-alpha (PDGFRα). Both pentamer and trimer need to interact with the glycoprotein B (gB), the fusogenic protein, to trigger the virus and host cell membrane fusion.
  • Clinical and Translational Updates

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