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Your Position: Start > Antibody > Envelope protein E/E protein (JEV) > ENE-MY2024

Monoclonal Anti-Japanese encephalitis virus Envelope protein E Antibody, Human IgG1 (1D6) (MALS verified)

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  • Source

    Monoclonal Anti-Japanese encephalitis virus Envelope protein E Antibody, Human IgG1 (1D6) is a chimeric monoclonal antibody recombinantly expressed from HEK293, which combines the variable region of a mouse monoclonal antibody with Human constant domain.

  • Clone

    1D6

  • Species

    Mouse

  • Isotype

    Human IgG1 | Human Kappa

  • Conjugate

    Unconjugated

  • Antibody Type

    Recombinant Monoclonal

  • Reactivity

    Virus

  • Immunogen

    Recombinant Japanese encephalitis virus (strain SA-14) Envelope protein E is expressed from human 293 cells.

  • Specificity

    Specifically recognizes Japanese encephalitis virus (strain SA-14) Envelope protein E.

  • Application
    ApplicationRecommended Usage
    ELISA0.1-50 ng/mL
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Purification

    Protein A purified/ Protein G purified

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Envelope protein E/E protein (JEV) SDS-PAGE

Monoclonal Anti-Japanese encephalitis virus Envelope protein E Antibody, Human IgG1 (1D6) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS
Envelope protein E/E protein (JEV) MALS images

The purity of Monoclonal Anti-Japanese encephalitis virus Envelope protein E Antibody, Human IgG1 (1D6) (Cat. No. ENE-MY2024) is more than 90% and the molecular weight of this protein is around 135-160 kDa verified by SEC-MALS.

Bioactivity-ELISA
 Envelope protein E/E protein (JEV) ELISA

Immobilized Japanese encephalitis virus (strain SA-14) Envelope protein E, His Tag (MALS verified) (Cat. No. ENE-J52H5) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Japanese encephalitis virus Envelope protein E Antibody, Human IgG1 (1D6) (Cat. No. ENE-MY2024) with a linear range of 0.1-3 ng/mL (QC tested).

  • Background
    Japanese encephalitis B, a mosquito-borne flavivirus, or B encephalitis, is the most important cause of viral encephalitis in Asia in terms of frequency and severity. JEV envelope protein E is an important research target, binding to host cell surface receptors and mediating fusion between the virus and the cell membrane.
  • Clinical and Translational Updates

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