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Your Position: Start > Cell > IDH1(132H)-P2A-mGFP&Luc > CHEK-ATP199

HEK293/Human IDH1(132H)-P2A-mGFP&Luc Stable Cell Line

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  • Description
    The HEK293/Human IDH1(132H)-P2A-mGFP&Luc Stable Cell Line was engineered to not only express the receptor full length human IDH1 mutated to a Histidine (H) at amino acid 132 and mGFP by a viral P2A self-cleaving peptide, but also express the firefly luciferase reporter. The expression of mGFP characterizing the human IDH1 (132H) was confirmed by flow cytometry. The Luciferase activity was confirmed by the detection of luminescence signal.
  • Application

    •        Screen for human IDH1 inhibitor

  • Growth Properties
    Adherent
  • Selection Marker
    Puromycin (2 μg/mL) + Hygromycin (20 μg/mL)
  • Culture Medium
    DMEM + 10% FBS
  • Freeze Medium
    Serum-free cell cryopreservation medium
  • Quantity
    1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium
  • Storage
    Frozen in liquid nitrogen.
  • Mycoplasma Testing
    Negative
  • Sterility Testing
    Negative
  • Instructions for Use
    See data sheet for detailed culturing and assay protocol.
Receptor Assay
 IDH1(132H)-P2A-mGFP&Luc FACS

Expression analysis of human IDH1 (132H) on HEK293/Human IDH1(132H)-P2A-mGFP&Luc Stable Cell Line by FACS.
The expression of mGFP characterizing the human IDH1 (132H) was detected by flow cytometry.

Application
 IDH1(132H)-P2A-mGFP&Luc APPLICATION

Expression analysis of Luciferase on HEK293/Human IDH1(132H)-P2A-mGFP&Luc Stable Cell Line (RLU).
The luminescence signal of HEK293/Human IDH1(132H)-P2A-mGFP&Luc Stable Cell Line were detected by incubating with the luciferase substrate. The RLU value was approximately 5.84 x10^7 at the density of 1x10^4 cells/well.

 IDH1(132H)-P2A-mGFP&Luc APPLICATION

Luciferase activity of HEK293/Human IDH1(132H)-P2A-mGFP&Luc Stable Cell Line (FOLD).
The luminescence signal of HEK293/Human IDH1(132H)-P2A-mGFP&Luc Stable Cell Line were detected by incubating with the luciferase substrate. The induction fold was approximately 2171.46.

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  • Permits & Restrictions
    This cell line is provided for research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. You are not allowed to share, distribute, sell, modify, sublicense, or otherwise make this cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. AcroBiosystems does not warrant the suitability of this cell line for any particular use, and does not accept any liability in connection with the handling or use of this cell line.
  • Clinical and Translational Updates

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Voraussichtlicher Liefertermin für vorrätige Produkte: 2 Arbeitstage

Price(EUR) : €3540.00

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