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Your Position: Start > Protein > CD1d&B2M > CDM-H52H5

Human CD1d&B2M Monomer Protein (Peptide free, MALS verified)

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  • Source
    Human CD1d&B2M Monomer Protein(CDM-H52H5) is expressed from human 293 cells (HEK293). It contains AA Glu 20 - Ser 301 (CD1d) & Ile 21 - Met 119 (B2M) (Accession # P15813 (CD1d) & P61769 (B2M)).
    Predicted N-terminus: Glu 20 & Ile 21
  • Molecular Characterization

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 37.3 kDa and 15.7 kDa. The protein migrates as 55-60 kDa and 15-16 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 0.1 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
CD1d&B2M SDS-PAGE

Human CD1d&B2M Monomer Protein on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS
CD1d&B2M MALS images

The purity of Human CD1d&B2M Monomer Protein (Cat. No. CDM-H52H5) is more than 90% and the molecular weight of this protein is around 50-75 kDa verified by SEC-MALS.

Bioactivity-ELISA
 CD1d&B2M ELISA

Immobilized Human CD1d&B2M Monomer Protein (Cat. No. CDM-H52H5) at 1 μg/mL (100 μL/well) can bind Human CD1d Antibody, Mouse IgG2B with a linear range of 0.05-2 ng/mL (QC tested).

  • Clinical and Translational Updates

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