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Your Position: Start > Antibody > Glycoprotein/G Protein (RABV) > GLN-M645

Monoclonal Anti-Glycoprotein G(Lyssavirus rabies) Antibody, Human IgG1 (4A7) (MALS verified)

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  • Source

    Monoclonal Anti-Glycoprotein G(Lyssavirus rabies) Antibody, Human IgG1 (4A7) is a chimeric monoclonal antibody recombinantly expressed from HEK293, which combines the variable region of a mouse monoclonal antibody with Human constant domain.

  • Clone

    4A7

  • Isotype

    Human IgG1 | Human Kappa

  • Conjugate

    Unconjugated

  • Antibody Type

    Recombinant Monoclonal

  • Reactivity

    Virus

  • Immunogen

    Recombinant Rabies virus Glycoprotein G derived from Baculovirus-Insect cells.

  • Specificity

    This product is a specific antibody specifically reacts with Rabies virus Glycoprotein G.

  • Application
    ApplicationRecommended Usage
    ELISA0.1-75 ng/mL 
  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Purification

    Protein A purified/ Protein G purified

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Glycoprotein/G Protein (RABV) SDS-PAGE

Monoclonal Anti-Glycoprotein G(Lyssavirus rabies) Antibody, Human IgG1 (4A7) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS
Glycoprotein/G Protein (RABV) MALS images

The purity of Monoclonal Anti-Glycoprotein G(Lyssavirus rabies) Antibody, Human IgG1 (4A7) (Cat. No. GLN-M645) is more than 90% and the molecular weight of this protein is around 135-165 kDa verified by SEC-MALS.

Bioactivity-ELISA
 Glycoprotein/G Protein (RABV) ELISA

Immobilized Rabies virus Glycoprotein G, His Tag (Cat. No. RAG-V55H5) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Glycoprotein G(Lyssavirus rabies) Antibody, Human IgG1 (4A7) (Cat. No. GLN-M645) with a linear range of 0.1-1 ng/mL (QC tested).

Bioactivity-SPR
 Glycoprotein/G Protein (RABV) SPR

Monoclonal Anti-Glycoprotein G(Lyssavirus rabies) Antibody, Human IgG1 (4A7) (Cat. No. GLN-M645) captured on Protein A Chip can bind Rabies virus Glycoprotein G, His Tag (Cat. No. RAG-V55H5) with an affinity constant of 1.25 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).

  • Background
    Rabies virus (RABV), scientific name Rabies lyssavirus, is a deadly neurotropic virus that causes rabies in humans and animals. Rabies virus has an extremely wide host range and its transmission most often occur through the saliva of animals. Without intervention prior to disease progression, rabies has the highest case fatality of any infectious disease. RABV contains a single-stranded negative-sense RNA genome that encodes five structural proteins: nucleoprotein (N), phosphoprotein (P), matrix protein (M), glycoprotein (G), and RNA-dependent RNA polymerase (L). Among these viral proteins, the RABV glycoprotein (RABV-G) is a pivotal player mediating virus entry and the major target of neutralizing antibodies, thus a key factor for vaccine and drug design.
  • Clinical and Translational Updates

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