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Your Position: Start > Protein > Fc gamma RIV / CD16-2 > FC4-M82E8

Biotinylated Mouse Fc gamma RIV / CD16-2 Protein, His,Avitag™ (HPLC & BLI verified)

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  • Synonym
    Fc gamma RIV,CD16-2,Fcgr4
  • Source
    Biotinylated Mouse Fc gamma RIV, His,Avitag (BLI verified)(FC4-M82E8) is expressed from human 293 cells (HEK293). It contains AA Gly 21 - Gln 203 (Accession # A0A0B4J1G0-1).
    Predicted N-terminus: Gly 21
  • Molecular Characterization
    Fc gamma RIV / CD16-2 Structure

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™)

    The protein has a calculated MW of 24.6 kDa. The protein migrates as 30-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 12 months under sterile conditions after reconstitution.
SDS-PAGE
Fc gamma RIV / CD16-2 SDS-PAGE

Biotinylated Mouse Fc gamma RIV, His,Avitag (BLI verified) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-HPLC
Fc gamma RIV / CD16-2 SEC-HPLC

The purity of Biotinylated Mouse Fc gamma RIV, His,Avitag (BLI verified) (Cat. No. FC4-M82E8) was greater than 85% as determined by SEC-HPLC.

Bioactivity-ELISA
 Fc gamma RIV / CD16-2 ELISA

Immobilized FMC63 at 5 μg/mL (100 μL/well) can bind Biotinylated Mouse Fc gamma RIV, His,Avitag (BLI verified) (Cat. No. FC4-M82E8) with a linear range of 0.3-20 ng/mL (Routinely tested).

Bioactivity-SPR
 Fc gamma RIV / CD16-2 SPR

Biotinylated Mouse Fc gamma RIV, His,Avitag (BLI verified) (Cat. No. FC4-M82E8) captured on Biotin CAP - Series S sensor Chip can bind Herceptin with an affinity constant of 0.186 μM as determined in a SPR assay (Biacore 8K) (Routinely tested).

 Fc gamma RIV / CD16-2 SPR

Biotinylated Mouse Fc gamma RIV, His,Avitag (BLI verified) (Cat. No. FC4-M82E8) captured on Biotin CAP - Series S sensor Chip can bind Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a (Clone: OKT3), Ultra-low endotoxin (Cat. No. CDE-M120a) with an affinity constant of 31.7 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).

Bioactivity-BLI
 Fc gamma RIV / CD16-2 BLI

Biotinylated Mouse Fc gamma RIV, His,Avitag (BLI verified) (Cat. No. FC4-M82E8) on SA Biosensor, can bind Rituximab with an affinity constant of 0.394 μM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).

  • Background
    FcgR4(Low affinity immunoglobulin gamma Fc region receptor IV) is also known as CD16-2, FcgammaRIV, receptor for the Fc region of immunoglobulin gamma . Also acts as a receptor for the Fc region of immunoglobulin epsilon . Binds with intermediate affinity to both IgG2a and IgG2b . Does not display binding to IgG1 or IgG3. Plays a role in promoting bone resorption by enhancing osteoclast differentiation following binding to IgG2a. Binds with low affinity to both the a and b allotypes of IgE. Has also been shown to bind to IgE allotype a only but not to allotype b. Binding to IgE promotes macrophage-mediated phagocytosis, antigen presentation to T cells, production of proinflammatory cytokines and the late phase of cutaneous allergic reactions
  • Clinical and Translational Updates

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  • Number of Drugs in Clinical Trials:4 Details
  • Latest Research Phase:Phase 2 Clinical

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