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Your Position: Start > Antibody > MMAE > MME-BLS104

Biotinylated Monoclonal Anti-MMAE&MMAF Antibody, Mouse IgG1 (MALS verified)

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  • Source

    Biotinylated Monoclonal Anti-MMAE&MMAF Antibody, Mouse IgG1 is a Mouse monoclonal antibody recombinantly expressed from HEK293 cells.

  • Species

    Mouse

  • Isotype

    Mouse IgG1 | Mouse kappa

  • Conjugate

    Biotin

  • Antibody Type

    Recombinant Monoclonal

  • Reactivity

    Chemical

  • Immunogen

    MMAE.

  • Specificity

    Specifically recognizes the target-MMAE&MMAF.

  • Application
    ApplicationRecommended Usage
    ELISA2-250 ng/mL 
  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Purification

    Protein A purified/ Protein G purified

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
MMAE SDS-PAGE

Biotinylated Monoclonal Anti-MMAE&MMAF Antibody, Mouse IgG1 on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS
MMAE MALS images

The purity of Biotinylated Monoclonal Anti-MMAE&MMAF Antibody, Mouse IgG1 (Cat. No. MME-BLS104) is more than 90% and the molecular weight of this protein is around 135-165 kDa verified by SEC-MALS.

Bioactivity-ELISA
 MMAE ELISA

Immobilized Disitamab Vedotin (RC48) at 1 μg/mL (100 μL/well) can bind Biotinylated Monoclonal Anti-MMAE&MMAF Antibody, Mouse IgG1 (Cat. No. MME-BLS104) with a linear range of 2-31 ng/mL (QC tested).

 MMAE ELISA

Immobilized Biotinylated Monoclonal Anti-MMAE&MMAF Antibody, Mouse IgG1 (Cat. No. MME-BLS104) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Disitamab Vedotin (RC48) with a linear range of 0.0078-0.125 ng/mL (Routinely tested).

  • Background
    Monomethyl auristatin E (MMAE) is a synthetic derivative of dolastatin 10 and functions as a potent mitotic inhibitor by inhibiting tubulin polymerization. MMAE is widely used as a cytotoxic component of antibody-drug conjugates (ADCs) to treat several different cancer types. Anti-MMAE antibody is a Mouse monoclonal antibody. This antibody has been shown to work in applications such as: PK, PD, Immunoassay and ELISA.
  • Clinical and Translational Updates

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