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Human CA125 / MUC16 (13810-14451) Protein, His Tag (MALS verified)

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  • Synonym
    CA125,CA-125,CA125MUC-16,FLJ14303,MUC16,mucin 16,mucin-16
  • Source
    Human CA125 (13810-14451), His Tag(CA5-H52H6) is expressed from human 293 cells (HEK293). It contains AA Pro 13810 - Pro 14451 (Accession # Q8WXI7-1).
    Predicted N-terminus: Pro 13810
  • Molecular Characterization
    CA125 Structure

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 73.7 kDa. The protein migrates as 90-130 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 0.1 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
CA125 SDS-PAGE

Human CA125 (13810-14451), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS
CA125 MALS images

The purity of Human CA125 (13810-14451), His Tag (Cat. No. CA5-H52H6) is more than 90% and the molecular weight of this protein is around 85-115 kDa verified by SEC-MALS.

Bioactivity-ELISA
 CA125 ELISA

Immobilized Human CA125 (13810-14451), His Tag (Cat. No. CA5-H52H6) at 5 μg/mL (100 μL/well) can bind Human Mesothelin (296-580), Fc Tag (Cat. No. MSN-H5253) with a linear range of 0.2-10 ng/mL (QC tested).

Bioactivity-SPR
 CA125 SPR

Human Mesothelin (296-580), His Tag, premium grade (Cat. No. MSN-H522a) immobilized on CM5 Chip can bind Human CA125 (13810-14451), His Tag (Cat. No. CA5-H52H6) with an affinity constant of 1.82 μM as determined in a SPR assay (Biacore 8K) (Routinely tested).

  • Background
    The CA125, also known as the MUC16, is a mucin protein that may be found in type I transmembrane or secreted forms that are used monitor the progress of epithelial ovarian cancer therapy. The CA 125 molecule is almost certainly a glycoprotein with a predominance of O-linkages. It is heterogeneous with regard to both size and charge, most likely due to continuous deglycosylation of side chains during its life-span in bodily fluids. It exists as a very large complex (perhaps as much as 4 million daltons) under natural conditions.
  • Clinical and Translational Updates

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