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Human GAS6 (279-678) Protein, His Tag (MALS verified)

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  • Synonym
    GAS6,GAS-6,AXLLG
  • Source
    Human GAS6, His Tag(GA6-H5249) is expressed from human 293 cells (HEK293). It contains AA Asp 279 - Ala 678 (Accession # Q14393-2).
    Predicted N-terminus: Asp 279
  • Molecular Characterization
    GAS6 Structure

    This protein carries a polyhistidine tag at the N-terminus.

    The protein has a calculated MW of 46.0 kDa. The protein migrates as 45-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
GAS6 SDS-PAGE

Human GAS6, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
GAS6 MALS images

The purity of Human GAS6, His Tag (Cat. No. GA6-H5249) is more than 90% and the molecular weight of this protein is around 44-58 kDa verified by SEC-MALS.

Bioactivity-ELISA
 GAS6 ELISA

Immobilized Human GAS6, His Tag (Cat. No. GA6-H5249) at 5 μg/mL (100 μL/well) can bind Human Axl, Fc Tag (Cat. No. AXL-H5253) with a linear range of 0.1-10 ng/mL (QC tested).

Bioactivity-SPR
 GAS6 SPR

Human Axl, His Tag (Cat. No. AXL-H5226) immobilized on CM5 Chip can bind Human GAS6, His Tag (Cat. No. GA6-H5249) with an affinity constant of 3.4 nM as determined in SPR assay (Biacore 8K) (Routinely tested).

 GAS6 SPR

Human MERTK, His Tag (Cat. No. MEK-H52H6) immobilized on CM5 Chip can bind Human GAS6, His Tag (Cat. No. GA6-H5249) with an affinity constant of 50.9 nM as determined in SPR assay (Biacore 8K) (Routinely tested).

  • Background
    GAS6 (Growth arrest-specific protein 6) is also known as AXL receptor tyrosine kinase ligand, AXLLG. Ligand for tyrosine-protein kinase receptors AXL, TYRO3 and MER whose signaling is implicated in cell growth and survival, cell adhesion and cell migration. GAS6/AXL signaling plays a role in various processes such as endothelial cell survival during acidification by preventing apoptosis, optimal cytokine signaling during human natural killer cell development, hepatic regeneration, gonadotropin-releasing hormone neuron survival and migration, platelet activation, or regulation of thrombotic responses.
  • Clinical and Translational Updates

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