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Your Position: Start > Protein > OX40 > OX0-H5224

Human OX40 / TNFRSF4 / CD134 Protein, His Tag (MALS verified)

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  • Synonym
    TNFRSF4,OX40,CD134,OX40L receptor,ACT35,TXGP1L
  • Source
    Human OX40 Protein, His Tag(OX0-H5224) is expressed from human 293 cells (HEK293). It contains AA Leu 29 - Ala 216 (Accession # P43489-1).
    Predicted N-terminus: Leu 29
  • Molecular Characterization
    OX40 Structure

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 22.0 kDa. The protein migrates as 35-45 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
OX40 SDS-PAGE

Human OX40 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS
OX40 MALS images

The purity of Human OX40 Protein, His Tag (Cat. No. OX0-H5224) is more than 85% and the molecular weight of this protein is around 32-44 kDa verified by SEC-MALS.

Bioactivity-ELISA
 OX40 ELISA

Immobilized Human OX40 Protein, His Tag (Cat. No. OX0-H5224) at 2 μg/mL (100 μL/well) can bind Human OX40 Ligand, Fc Tag, premium grade (Cat. No. OXL-H5266) with a linear range of 0.1-2 ng/mL (QC tested).

 OX40 ELISA

Immobilized Human OX40 Protein, His Tag (Cat. No. OX0-H5224) at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-Human OX40 Antibody, Human IgG1 with a linear range of 0.1-2 ng/mL (Routinely tested).

 OX40 ELISA

Immobilized Human OX40 Protein, His Tag (Cat. No. OX0-H5224) at 2 μg/mL, add increasing concentrations of CTLA-4 x OX40 bispecific antibody in 50% Human serum and then add Biotinylated Human CTLA-4, Fc,Avitag (Cat. No. CT4-H82F3) at 0.2 μg/mL. Detection was performed using HRP-conjugated streptavidin with sensitivity of 4 ng/mL (Intact assay, Routinely tested).

Bioactivity-BLI
 OX40 BLI

Loaded Human OX40 Ligand, His Tag (Cat. No. OXL-H52Q8) on AR2G Biosensor, can bind Human OX40 Protein, His Tag (Cat. No. OX0-H5224) with an affinity constant of 0.15 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

 OX40 BLI

Loaded Anti-OX40 MAb(Human IgG1) on AHC Biosensor, can bind Human OX40 Protein, His Tag (Cat. No. OX0-H5224) with an affinity constant of 6.03 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

  • Background
    Tumor necrosis factor receptor superfamily member 4 (TNFRSF4) is also known as ACT35 antigen, OX40L receptor, TAX transcriptionally-activated glycoprotein 1 receptor, CD antigen CD134, OX40. OX40 / TNFRSF4 contains four TNFR-Cys repeats. TNFRSF4 is receptor for TNFSF4 / OX40L / GP34 and can interacts with TRAF2, TRAF3 and TRAF5.
  • Clinical and Translational Updates

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