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Your Position: Start > Protein > Activin RIIB > ACB-H82F3

Biotinylated Human Activin RIIB Protein, Fc,Avitag™ (MALS verified)

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  • Synonym
    ACVR2B,ACTRIIB,MGC116908
  • Source
    Biotinylated Human Activin RIIB Protein, Fc,Avitag(ACB-H82F3) is expressed from human 293 cells (HEK293). It contains AA Ser 19 - Thr 137 (Accession # Q13705-1 ).
    Predicted N-terminus: Ser 19
  • Molecular Characterization
    Activin RIIB Structure

    This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 41.8 kDa. The protein migrates as 50-60 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Activin RIIB SDS-PAGE

Biotinylated Human Activin RIIB Protein, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS
Activin RIIB MALS images

The purity of Biotinylated Human Activin RIIB Protein, Fc,Avitag (Cat. No. ACB-H82F3) is more than 85% and the molecular weight of this protein is around 90-120 kDa verified by SEC-MALS.

Bioactivity-ELISA
 Activin RIIB ELISA

Immobilized Human Latent Activin A, His Tag (Cat. No. ACA-H424x) at 5 μg/mL (100 μL/well) can bind Biotinylated Human Activin RIIB Protein, Fc,Avitag (Cat. No. ACB-H82F3) with a linear range of 2-31 ng/mL (QC tested).

Bioactivity-SPR
 Activin RIIB SPR

Biotinylated Human Activin RIIB Protein, Fc,Avitag (Cat. No. ACB-H82F3) immobilized on SA Chip can bind Human Latent Activin A, His Tag (Cat. No. ACA-H424x) with an affinity constant of 46.8 pM as determined in a SPR assay (Biacore 8K) (Routinely tested).

  • Background
    Activin receptor type-2B (ACVR2B) is also known as ActR-IIB and MGC116908, ACVR2B is an activin type 2 receptor. Activins are dimeric growth and differentiation factors which belong to the transforming growth factor-beta (TGF-beta) superfamily of structurally related signaling proteins. Activins signal through a heteromeric complex of receptor serine kinases which include at least two type I (I and IB) and two type II (II and IIB) receptors. These receptors are all transmembrane proteins, composed of a ligand-binding extracellular domain with cysteine-rich region, a transmembrane domain, and a cytoplasmic domain with predicted serine/threonine specificity. Type I receptors are essential for signaling; and type II receptors are required for binding ligands and for expression of type I receptors. Type I and II receptors form a stable complex after ligand binding, resulting in phosphorylation of type I receptors by type II receptors. Type II receptors are considered to be constitutively active kinases. This gene encodes activin A type IIB receptor, which displays a 3- to 4-fold higher affinity for the ligand than activin A type II receptor. Defects in ACVR2B are the cause of visceral heterotaxy autosomal type 4 (HTX4).
  • Clinical and Translational Updates

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  • Number of Drugs in Clinical Trials:2 Details
  • Latest Research Phase:Phase 2 Clinical

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