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Your Position: Start > Protein > DNA polymerase I > DNI-B5113

Bst DNA Polymerase

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  • Product Details
    Bst DNA Polymerase is a recombinant form of Bacillus stearothermophilus DNA Polymerase, Large Fragment produced in Escherichia coli. Bst DNA Polymerase contains 5´→3´ DNA polymerase activity with either DNA or RNA templates and strong strand displacement activity, but lacks 5´→3´ and 3´→5´ exonuclease activity.
  • Application

    Isothermal amplification (LAMP, RT-LAMP)
    DNA sequencing of rich GC sequence
    Rapid sequencing of DNA templates (ng)

  • Unit Definition
    One unit is defined as the amount of enzyme that will incorporate 25 nmol of dNTP into acid insoluble material in 30 minutes at 65°C.
  • Purity

    >90% as determined by SDS-PAGE.

  • Enzyme Activity
    >8 U/μL
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Formulation

    Supplied as 0.2 μm filtered solution in 10 mM Tris-HCl, pH7.1 with glycerol as protectant.

    Contact us for customized product form or formulation.

  • Shipping

    This product is supplied and shipped with blue ice, please inquire the shipping cost.

  • Storage

    This product is stable after storage at:

    1. The product MUST be stored at -20°C or lower upon receipt.
    2. -20°C for 3 months under sterile conditions.
SDS-PAGE
DNA polymerase I SDS-PAGE

The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

Bioactivity
 DNA polymerase I ENZYME

One unit is defined at the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 65°C (QC tested).

  • Clinical and Translational Updates

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