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Your Position: Start > Kits > Kanamycin > RES-A078

resDetect™ Kanamycin ELISA Kit

For research use only.

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    Materials Provided
    IDComponentsSize
    RES078-C01Kanamycin Coated Plate1 plate
    RES078-C02Kanamycin Standard81ng/mL×1.9mL
    RES078-C03HRP-Anti-Kanamycin Antibody6mL
    RES078-C041×Dilution Buffer50mL
    RES078-C0520xWashing Buffer 50mL
    RES078-C06Substrate Solution12mL
    RES078-C07Stop Solution7mL
  • Background
    Residues of kanamycin are prone to occur in the production process of biological products, which can easily lead to abnormal reactions in the human body. Therefore, the residual amount of kanamycin in biological products or semi-finished products of biological products should be strictly controlled.
  • Application

    The kit is calibrated against the USP standards. It's developed for the detection and quantitative determination of Kanamycin residues in plasmid DNA raw materials and proteins for CGT, vaccine and other biological drugs.

    It is for research use only.

  • Reconstitution
    Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
  • Storage
    1. Unopened kit should be stored at 2℃-8℃ upon receiving.

    2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.

    3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

  • Assay Principles
    The Kanamycin quantitative detection kit adopts the indirect competitive ELISA method, and the pre-coated conjugated kanamycin antigen on the microstrip competes with the residual kanamycin in the sample to bind the enzyme-labeled anti-kanamycin monoclonal antibody, and then uses a microplate reader to detect the absorbance value by adding TMB substrate, and the absorbance value is negatively correlated with the content of kanamycin in the sample. The kit takes only about one hour and 20 minutes to operate and has a linear range of 0.5 ng/mL to 40.5 ng/mL.
Typical Data Please refer to DS document for the assay protocol.
 Kanamycin TYPICAL DATA

Serial dilutions of kanamycin (from 40.5 ng/mL to 0.5 ng/mL) was added into kanamycin: anti-kanamycin binding reactions. The assay was performed according to the protocol dscribed below. Background was subtracted from data points prior to log transformation and curve fitting (QC tested).

  • Clinical and Translational Updates

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Price(EUR) : €610.00

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