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Biotinylated Cynomolgus / Rhesus macaque CTLA-4 Protein, Fc,Avitag™ (MALS verified)

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  • Synonym
    CTLA4,CD152
  • Source
    Biotinylated Cynomolgus / Rhesus macaque CTLA-4 Protein, Fc,Avitag(CT4-C82F3) is expressed from human 293 cells (HEK293). It contains AA Ala 37 - Ser 160 (Accession # G7PL88-1).
    Predicted N-terminus: Ala 37
  • Molecular Characterization
    CTLA-4 Structure

    This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 41.4 kDa. The protein migrates as 47-55 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
CTLA-4 SDS-PAGE

Biotinylated Cynomolgus / Rhesus macaque CTLA-4 Protein, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS
CTLA-4 MALS images

The purity of Biotinylated Cynomolgus / Rhesus macaque CTLA-4 Protein, Fc,Avitag (Cat. No. CT4-C82F3) is more than 90% and the molecular weight of this protein is around 85-105 kDa verified by SEC-MALS.

Bioactivity-ELISA
 CTLA-4 ELISA

Immobilized Monoclonal Anti-Human CTLA-4 Antibody, Human IgG1 (Cat. No. CT4-M1203) at 2 μg/mL (100 μL/well) can bind Biotinylated Cynomolgus / Rhesus macaque CTLA-4 Protein, Fc,Avitag (Cat. No. CT4-C82F3) with a linear range of 0.1-2 ng/mL (QC tested).

 CTLA-4 ELISA

Immobilized Cynomolgus / Rhesus macaque B7-1, Fc Tag (Cat. No. CD0-C5252) at 2 μg/mL (100 μL/well) can bind Biotinylated Cynomolgus / Rhesus macaque CTLA-4 Protein, Fc,Avitag (Cat. No. CT4-C82F3) with a linear range of 0.1-2 ng/mL (Routinely tested).

  • Background
    CTLA-4 (Cytotoxic T-Lymphocyte Antigen 4) is also known as CD152 (Cluster of differentiation 152), is a protein receptor that downregulates the immune system. CTLA4 is a member of the immunoglobulin superfamily, which is expressed on the surface of Helper T cells and transmits an inhibitory signal to T cells. The protein contains an extracellular V domain, a transmembrane domain, and a cytoplasmic tail. Alternate splice variants, encoding different isoforms. CTLA4 is similar to the T-cell co-stimulatory protein, CD28, and both molecules bind to CD80 and CD86, also called B7-1 and B7-2 respectively, on antigen-presenting cells. CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Intracellular CTLA4 is also found in regulatory T cells and may be important to their function. Fusion proteins of CTLA4 and antibodies (CTLA4-Ig) have been used in clinical trials for rheumatoid arthritis.
  • Clinical and Translational Updates

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  • Latest Research Phase:Approved

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