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Your Position: Start > Protein > PDE4A > PDA-H55H3

Human PDE4A Protein, His Tag (MALS verified)

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  • Synonym
    phosphodiesterase 4A,cAMP-specific 3',5'-cyclic phosphodiesterase 4A
  • Source
    Human PDE4A Protein, His Tag(PDA-H55H3) is expressed from Baculovirus-Insect cells. It contains AA Pro 331 - Met 723 (Accession # P27815-1).
    Predicted N-terminus: Met
  • Molecular Characterization
    PDE4A Structure

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 47.1 kDa. The protein migrates as 45-52 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Supplied as 0.2 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with glycerol as protectant.

    Contact us for customized product form or formulation.

  • Shipping

    This product is supplied and shipped with dry ice, please inquire the shipping cost.

  • Storage

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -70°C or lower upon receipt;
    2. -70°C for 3 months under sterile conditions.
SDS-PAGE
PDE4A SDS-PAGE

Human PDE4A Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS
PDE4A MALS images

The purity of Human PDE4A Protein, His Tag (Cat. No. PDA-H55H3) is more than 90% and the molecular weight of this protein is around 48-68 kDa verified by SEC-MALS.

Bioactivity

Measured by its ability to convert cAMP to 5'AMP. The specific activity is >140,000 pmol/min/μg (QC tested).

  • Background
    cAMP-specific 3',5'-cyclic phosphodiesterase 4A (PDE4A) is an enzyme that in humans is encoded by the PDE4A gene. It belongs to the PDE family who hydrolyze 3′-5′-cyclic adenosine monophosphate (cyclic AMP) to 5′-adenosine monophosphate (AMP). PDE enzyme family plays a pivotal role in regulating levels of the second messenger cAMP. Consequently, PDE4 inhibitors have been investigated as a therapeutic strategy to enhance cAMP signaling in a broad range of diseases, including several types of cancers, as well as in various neurologic, dermatological, and inflammatory diseases.
  • Clinical and Translational Updates

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Price(EUR) : €350.00

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