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Your Position: Start > Antibody > A17L (MPXV) > A1L-M698

Monoclonal Anti-Monkeypox virus (strain Zaire-96-I-16) A17L Antibody, Human IgG1 (1B4)

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  • Source

    Monoclonal Anti-Monkeypox virus (strain Zaire-96-I-16) A17L Antibody, Human IgG1 (1B4) is a chimeric monoclonal antibody recombinantly expressed from HEK293, which combines the variable region of a mouse monoclonal antibody with Human constant domain.

  • Clone

    1B4

  • Isotype

    Human IgG1 | Human Kappa

  • Conjugate

    Unconjugated

  • Antibody Type

    Recombinant Monoclonal

  • Reactivity

    Virus

  • Immunogen

    Recombinant Monkeypox virus (strain Zaire-96-I-16) A17L Protein is expressed from human 293 cells.

  • Specificity

    Specifically recognizes Monkeypox virus (strain Zaire-96-I-16) A17L.

  • Application
    ApplicationRecommended Usage
    ELISA0.06-15 ng/mL 
  • Purity

    >90% as determined by SDS-PAGE.

  • Purification

    Protein A purified/ Protein G purified

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
A17L (MPXV) SDS-PAGE

Monoclonal Anti-Monkeypox virus (strain Zaire-96-I-16) A17L Antibody, Human IgG1 (1B4) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

Bioactivity-ELISA
 A17L (MPXV) ELISA

Immobilized Monkeypox virus (strain Zaire-96-I-16) A17L Protein, His Tag (Cat. No. A1L-M52H3) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Monkeypox virus (strain Zaire-96-I-16) A17L Antibody, Human IgG1 (1B4) (Cat. No. A1L-M698) with a linear range of 0.06-2 ng/mL (QC tested).

Bioactivity-SPR
 A17L (MPXV) SPR

Monoclonal Anti-Monkeypox virus (strain Zaire-96-I-16) A17L Antibody, Human IgG1 (1B4) (Cat. No. A1L-M698) captured on Protein A Chip can bind Monkeypox virus (strain Zaire-96-I-16) A17L Protein, His Tag (Cat. No. A1L-M52H3) with an affinity constant of 7.98 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).

  • Background
    Monkeypox is a rare zoonosis caused by monkeypox virus, which has become the most serious orthpoxvirus and consists of complex double stranded DNA. The pathogenesis of monkeypox is that the virus invades the body from respiratory mucosa, multiplies in lymphocytes, and incurs into blood producing transient venereal toxemia. after the virus multiplies in cells, the cells can invade the blood and propagate to the skin of the whole body, causing lesions. A17L is essential for an early step in virion morphogenesis.
  • Clinical and Translational Updates

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