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Your Position: Start > Kits > Glycoprotein E (VZV) > RAS-A185

Varicella Zoster Virus Glycoprotein E (VZV gE) ELISA Kit (For Vaccine Development)

For research use only.

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IDComponentsSize
RAS185-C01Pre-coated Anti-Glycoprotein E (VZV) Antibody Microplate1 plate
RAS185-C02Glycoprotein E (VZV) Standard30 μg
RAS185-C03HRP-Anti-Glycoprotein E (VZV) Antibody15 μg
RAS185-C0410xWashing Buffer 50 mL
RAS185-C052xDilution Buffer50 mL
RAS185-C06Substrate Solution12 mL
RAS185-C07Stop Solution7 mL
  • Background
    Varicella-zoster virus (VZV) the etiologic agent of chickenpox and herpes zoster [HZ], is highly contagious and still endemic worldwide. Glycoprotein E (gE) is one of the known glycoproteins (gB, gC, gE, gH, gI, gK, gI) of VZV that is most abundantly expressed on the surface of virus and infected cells, playing an important role in viral replication and cell-to-cell spread. The strongly immunogenic gE can provide strong IgG signal in body fluid, which makes it ideal to be developed as an antigen for analysis of Immunogenicity in the development of VZV vaccine.Therefore,It's helpful to develop the Varicella Zoster Virus Glycoprotein E (VZV gE) ELISA Kit to quantitative detection the VZV gE antigen in vaccine samples during the manufacture and quality control of vaccine development.
  • Application

    This kit is developed for quantitative detection of Varicella Zoster Virus Glycoprotein E (VZV gE) in vaccine samples.

    It is for research use only.

  • Reconstitution
    Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
  • Storage
    1. Unopened kit should be stored at 2℃-8℃ upon receiving.

    2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.

    3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

  • Assay Principles
    This assay kit employs a standard sandwich-ELISA format, providing a rapid detection of Glycoprotein E (VZV). The kit consists of Pre-coated Anti-Glycoprotein E (VZV) Antibody Microplate and Glycoprotein E (VZV) Standard,HRP-Anti-Glycoprotein E (VZV) Antibody and buffers.

    Your experiment will include 5 simple steps:

    a) Bring all reagents and samples to room temperature (20℃-25℃) before use.

    b) Add your sample to the plate, take the Glycoprotein E (VZV) as Control sample. The samples and Control sample are diluted by Dilution Buffer.

    c) Add the HRP-Anti-Glycoprotein E (VZV) Antibody diluted by Dilution Buffer to the plate.

    d) Wash the plate and add TMB.

    e) Stop the substrate reaction by adding diluted acid. Absorbance (OD) is calculated as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis. The OD Value reflects the amount of protein bound.

Typical Data Please refer to Ds document for the assay protocol.
 Glycoprotein E (VZV) TYPICAL DATA

For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The following example data is for reference only.

  • Clinical and Translational Updates

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Price(EUR) : €590.00

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