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Cynomolgus CD39 Protein, His Tag (active enzyme, MALS verified)

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  • Synonym
    CD39,ENTPD1,NTPDase 1,Entpd1,Ecto-ATPDase 1,Ecto-ATPase 1
  • Source
    Cynomolgus CD39 Protein, His Tag(CD9-C5PH3) is expressed from CHO cells. It contains AA Thr 60 - Val 500 (Accession # XP_015311944.1).
  • Molecular Characterization
    CD39 Structure

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 52.3 kDa. The protein migrates as 65-80 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Supplied as 0.2 μm filtered solution in 20 mM Tris, 150 mM NaCl, 20% Glycerol, pH8.0 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Shipping

    This product is supplied and shipped with dry ice, please inquire the shipping cost.

  • Storage

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -70°C or lower upon receipt;
    2. -70°C for 3 months under sterile conditions.
SDS-PAGE
CD39 SDS-PAGE

Cynomolgus CD39 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS
CD39 MALS images

The purity of Cynomolgus CD39 Protein, His Tag (Cat. No. CD9-C5PH3) is more than 85% and the molecular weight of this protein is around 65-85 kDa verified by SEC-MALS.

Bioactivity-ELISA
 CD39 ELISA

Immobilized Anti-CD39 Antibody, Human IgG1 at 1 μg/mL (100 μL/well) can bind Cynomolgus CD39 Protein, His Tag (Cat. No. CD9-C5PH3) with a linear range of 5-78 ng/mL (QC tested).

 CD39 ELISA

Immobilized Cynomolgus CD39 Protein, His Tag (Cat. No. CD9-C5PH3) at 1 μg/mL (100 μL/well) can bind Anti-CD39 Antibody, Human IgG1 with a linear range of 0.03-1 ng/mL (Routinely tested).

Bioactivity

Measured by its ability to hydrolyze the 5’-phosphate group from the substrate adenosine-5’-triphosphate (ATP). The specific activity is > 15,000 pmol/min/μg (QC tested).

  • Background
    CD39L1, also known as ENTPD2 and NTPDase2, is an ectonucleotidase belonging to the CD39 family. It is found on the surface of vascular adventitial cells and accessory vascular cells . CD39L1 is a Ca2+ and Mg2+ dependent enzyme that hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission. Hydrolyzes ADP only to a marginal extent. The order of activity with different substrates is ATP > GTP > CTP = ITP > UTP >> ADP = UDP. CD39L1 plays a role in regulating thrombosis and inflammation . It is considered to be a therapeutic target for thromboregulation and the treatment of vascular inflammation.
  • Clinical and Translational Updates

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