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Your Position: Start > Antibody > Anti-Human IgM > AHM-S300

Monoclonal Anti-Human IgM mu Chain Antibody-Acridinium ester (M3HC) (MALS verified)

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  • Source
    Monoclonal Anti-Human IgM mu Chain Antibody-Acridinium ester (M3HC) is a chimeric monoclonal antibody recombinantly expressed from human 293 cells (HEK293), which combines the variable region of a mouse monoclonal antibody with human IgG1 constant domain. The mouse monoclonal antibody is produced from a hybridoma resulting from fusion of SP2/0 myeloma and B-lymphocytes obtained from a mouse immunized with Anti-Human IgM.
  • Isotype
    Human IgG1/kappa
  • Specificity
    This product is a specific antibody specifically reacts with Anti-Human IgM.
  • Labeling
    Acridinium ester, can react with the primary amino group of protein. Under alkaline conditions, NHS is replaced as the leaving group, and the protein forms a stable amide bond with Acridinium ester.
  • Protein Ratio
    Passed as determined by binding MPCLIA.
  • Application
    MPCLIA
  • Purity

    >90% as determined by SDS-PAGE.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH6.3 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Anti-Human IgM SDS-PAGE

Monoclonal Anti-Human IgM mu Chain Antibody-Acridinium ester (M3HC) on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS
Anti-Human IgM MALS images

The purity of Monoclonal Anti-Human IgM mu Chain Antibody-Acridinium ester (M3HC) (Cat. No. AHM-S300) is more than 0.95 and the molecular weight of this protein is around 140-160 kDa verified by SEC-MALS.

Bioactivity-MPCLIA
 Anti-Human IgM MPCLIA

Immobilized 0.04 μg /Test of Biotinylated SARS-CoV-2 Spike RBD, His,Avitag(Cat. No.SPD-C82E9) to the Streptavidin-Magnetic Beads (recommended for MPCLIA) (Cat. No. MPC-A006) (10 μg beads/Test), incubated with 100 μL /Test of Anti-SARS-CoV-2 Spike RBD Neutralizing Antibody, Chimeric mAb, Human IgM (AM122) (Cat. No. SPD-M162) at increasing concentration coupled to the Monoclonal Anti-Human IgM mu Chain Antibody-Acridinium ester (M3HC) (MALS verified) (Cat. No. AHM-S300, 0.04 μg /Test). Detection was performed with sensitivity of 0.488 ng/mL in Magnetism particulate chemiluminescence immunoassay (MPCLIA) (KEYSMILE, SMART 6500S) (QC tested).

 Anti-Human IgM MPCLIA

The MPCLIA assay shows that Monoclonal Anti-Human IgM mu Chain Antibody-Acridinium ester (M3HC) (MALS verified) (Cat. No. AHM-S300) is stable at 37°C for 48 hours.

 Anti-Human IgM MPCLIA

The MPCLIA assay shows that Monoclonal Anti-Human IgM mu Chain Antibody-Acridinium ester (M3HC) (MALS verified) (Cat. No. AHM-S300) is stable after freezing and thawing 3 times.

  • Background
    The Anti-Human IgM-coupled Magnetic Beads are 2.8 µm superparamagnetic particles covalently coupled to a highly affinity Monoclonal anti-human IgM antibody. The beads can be used to capture the Human IgM in Chemiluminescence procedures. It has no-corss-reactivity with Human IgG, IgA or IgE. The Anti-Human IgM-coupled Magnetic Beads is easy to capture the human IgM, and the bounded antibody have no activity lost, this ready to use products could greatly save your protein coupling time and hassle, and help us get the best performance and highly reproducible results.
  • Clinical and Translational Updates

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