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Your Position: Start > Antibody > H3L (MPXV) > H3L-M611

Monoclonal Anti-Monkeypox-H3L Antibody, Human IgG1 (1C5)

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  • Source

    Monoclonal Anti-Monkeypox-H3L Antibody, Human IgG1 (1C5) is a chimeric monoclonal antibody recombinantly expressed from HEK293, which combines the variable region of a mouse monoclonal antibody with Human constant domain.

  • Clone

    1C5

  • Isotype

    Human IgG1 | Human Kappa

  • Conjugate

    Unconjugated

  • Antibody Type

    Recombinant Monoclonal

  • Reactivity

    Virus

  • Immunogen

    Recombinant Monkeypox virus (strain Zaire-96-I-16) H3L is expressed from human 293 cells.

  • Specificity

    Specifically recognizes Monkeypox virus (strain Zaire-96-I-16) H3L.

  • Application
    ApplicationRecommended Usage
    ELISA0.1-50 ng/mL 
  • Purity

    >90% as determined by SDS-PAGE.

  • Purification

    Protein A purified/ Protein G purified

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
H3L (MPXV) SDS-PAGE

Monoclonal Anti-Monkeypox-H3L Antibody, Human IgG1 (1C5) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

Bioactivity-ELISA
 H3L (MPXV) ELISA

Immobilized Monkeypox virus (strain Zaire-96-I-16) H3L, His Tag (Cat. No. H3L-M52H1) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Monkeypox-H3L Antibody, Human IgG1 (1C5) (Cat. No. H3L-M611) with a linear range of 0.1-2 ng/mL (QC tested).

Bioactivity-SPR
 H3L (MPXV) SPR

Monoclonal Anti-Monkeypox-H3L Antibody, Human IgG1 (1C5) (Cat. No. H3L-M611) captured on Protein A Chip can bind Monkeypox virus (strain Zaire-96-I-16) H3L, His Tag (Cat. No. H3L-M52H1) with an affinity constant of 0.194 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).

  • Background
    Monkeypox is a rare zoonosis caused by monkeypox virus, which has become the most serious orthpoxvirus and consists of complex double stranded DNA. The pathogenesis of monkeypox is that the virus invades the body from respiratory mucosa, multiplies in lymphocytes, and incurs into blood producing transient venereal toxemia. after the virus multiplies in cells, the cells can invade the blood and propagate to the skin of the whole body, causing lesions. H3L is similar to Vaccinia virus strain Copenhagen H3L heparin binding surface protein (Cop-H3L) surface membrane protein. There are research reports H3L induced transcriptional perturbations and injuries.
  • Clinical and Translational Updates

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