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The kit is developed for quantitative detection of natural and recombinant human TARC/CCL17 in serum, plasma and cell culture supernatants. It is for research use only.
It is for research use only.
| Analyte | CCL17 |
| Assay Type | Sandwich-ELISA |
| Reactivity | Human |
| Sensitivity | 3.0 pg/mL |
| Range | 31.25pg/mL-2000 pg/mL |
| Assay Time | 1.75 hr |
| Sample Type | Cell Culture Supernatants, Plasma, Serum. |
| Sample volume | 50 μL |
| Format | 96-wells plate breakable into 12 x 8 wells strips |
Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.
For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was calculated based on the results of the standard curve. The minimum detectable concentration of CCL17 is less than 3 pg/mL.
Serum – TARC/CCL17 concentrations were measured in 32 human serum samples, which were collected from healthy human subjects. The measurements of 32 samples are in the range of 1000-4000pg/mL and shown in the figure below.
3 types of materials were tested to observe if there were matrix effect (interference). If the concentration of hemoglobin (simulated hemolysis) is less than 3500 mg/dL(2%), the concentration of triglyceride (simulated lipid blood) is less than 3 mg/mL, testing results will not be affected.
High concentrations of human TARC/CCL17 serum samples were diluted with 1:2, 1:4, 1:8 and 1:16 ratios for gradient dilution to evaluate the linearity of the assay. In the serum samples, the average detection rate of TARC/CCL17 was 97.89%.
10 replicates of each of 3 samples containing different TARC/CCL17 concentrations were tested in one assay. Acceptable criteria: CV＜10%.
3 lots of samples containing different concentrations of TARC/CCL17 were tested in the independent assays. Acceptable criteria: CV<15%.
TARC/CCL17 was spiked into 3 human serum samples, and then analyzed. The average recovery of TARC/CCL17 for serum samples is 93.93%.
| ID | Components | Size |
| CEA029-C01 | Pre-coated Anti-CCL17 Antibody Microplate | 1 plate |
| CEA029-C02 | Human CCL17 Standard | 500 μL |
| CEA029-C03 | Biotin-Anti-CCL17 Antibody Con. Solution | 100 μL |
| CEA029-C04 | Biotin-Antibody Dilution Buffer | 8 mL |
| CEA029-C05 | Streptavidin-HRP Con. Solution | 0.5 mL |
| CEA029-C06 | Streptavidin-HRP Dilution Buffer | 15 mL |
| CEA029-C07 | 20× Washing Buffer | 50 mL |
| CEA029-C08 | Sample Dilution Buffer | 15 mL ×2 |
| CEA029-C09 | Substrate Solution | 12 mL |
| CEA029-C10 | Stop Solution | 6 mL |
Price(EUR) : €500.00
Price(EUR) :
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