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Cynomolgus KLRG1 Protein, His Tag (SPR verified)

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  • Synonym
    KLRG1
  • Source
    Cynomolgus KLRG1 Protein, His Tag(KL1-C5243) is expressed from human 293 cells (HEK293). It contains AA Leu 60 - Pro 189 (Accession # A0A2K5WAP9).
    Predicted N-terminus: His
  • Molecular Characterization
    KLRG1 Structure

    This protein carries a polyhistidine tag at the N-terminus

    The protein has a calculated MW of 16.8 kDa. The protein migrates as 18-21 kDa and 22-30 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
KLRG1 SDS-PAGE

Cynomolgus KLRG1 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

Bioactivity-SPR
 KLRG1 SPR

Human E-Cadherin, Fc Tag, premium grade (Cat. No. ECD-H5250) immobilized on CM5 Chip can bind Cynomolgus KLRG1 Protein, His Tag (Cat. No. KL1-C5243) with an affinity constant of 2.38 μM as determined in a SPR assay (Biacore 8K) (QC tested).

  • Background
    The co-inhibitory receptor killer-cell lectin like receptor G1 (KLRG1) is specifically expressed on NK cells and activated CD8+ T-cells and has been postulated to be a marker of senescence. KLRG1+ T cells are a major reason of chronic tissue damage in some autoimmune diseases such as systemic lupus erythematosus and rheumatoid arthritis. In tumors, tumor cells which express E-cadherin or N-cadherin bind to KLRG1 and inhibit the antitumor activity of T and NK cells. Thus, KLRG1 acts as an immunocheckpoint inhibitory receptor.
  • Clinical and Translational Updates

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Voraussichtlicher Liefertermin für vorrätige Produkte: 2 Arbeitstage

Price(EUR) : €350.00

Price(EUR) : €2330.00

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