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Your Position: Start > Protein > FCRL5 > FC5-H82E3

Biotinylated Human FCRL5 Protein, His,Avitag™ (MALS & SPR verified)

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  • Synonym
    BXMAS1, CD307, CD307e, FCRH5, IRTA2, PRO820
  • Source
    Biotinylated Human FCRL5, His,Avitag(FC5-H82E3) is expressed from human 293 cells (HEK293). It contains AA Gln 16 - Gly 851 (Accession # Q96RD9-1).
  • Molecular Characterization
    FCRL5 Structure

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™)

    The protein has a calculated MW of 95.0 kDa. The protein migrates as 105-125 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
FCRL5 SDS-PAGE

Biotinylated Human FCRL5, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
FCRL5 MALS images

The purity of Biotinylated Human FCRL5, His,Avitag (Cat. No. FC5-H82E3) is more than 85% and the molecular weight of this protein is around 100-120 kDa verified by SEC-MALS.

Bioactivity-SPR
 FCRL5 SPR

Biotinylated Human FCRL5 Protein, His,Avitag (Cat. No. FC5-H82E3) capture on NTA-Series S sensor chip can bind Ipilimumab with an affinity constant of 18.5 μM as determined in a SPR assay (Biacore 8K) (QC tested).

  • Background
    May be involved in B-cell development and differentiation in peripheral lymphoid organs and may be useful markers of B-cell stages. May have an immunoregulatory role in marginal zone B-cells.
  • Clinical and Translational Updates

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Drug Development Status

  • Number of Launched Drugs:0 Details
  • Number of Drugs in Clinical Trials:2 Details
  • Latest Research Phase:Phase 2 Clinical

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