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Your Position: Start > Antibody > IL-15 > IL5-M543

Anti-Human IL-15 (9B5G1) mAb, Mouse IgG1

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  • Source

    Anti-Human IL-15 (9B5G1) mAb, Mouse IgG1 is a Mouse monoclonal antibody recombinantly expressed from HEK293 cells.

  • Clone

    9B5G1

  • Species

    Mouse

  • Isotype

    Mouse IgG1 | Mouse Kappa

  • Conjugate

    Unconjugated

  • Antibody Type

    Recombinant Monoclonal

  • Reactivity

    Human

  • Immunogen

    Recombinant Human IL-15 is expressed from human HEK293 cells.

  • Specificity

    This product is a specific antibody specifically reacts with IL-15, Human. No cross-reactivity is detected with other human cytokines, including IL-2, IL-4, IL-6, IL-10, IL-21,GM-CSF,TNF-alpha and IFNγ.

  • Application
    ApplicationRecommended Usage
    ELISA1-250 ng/mL
  • Purity

    >95% as determined by SDS-PAGE.

  • Purification

    Protein A purified/ Protein G purified

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
IL-15 SDS-PAGE

Anti-Human IL-15 (9B5G1) mAb, Mouse IgG1 on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
 IL-15 ELISA

Immobilized Anti-Human IL-15 (9B5G1) MAb, Mouse IgG1 (Cat. No. IL5-M543) at 1 μg/mL (100 μL/well) can bind Biotinylated Human IL-15, Fc,Avitag (Cat. No. IL5-H82F3) with a linear range of 1-16 ng/mL (QC tested).

Bioactivity-SPR
 IL-15 SPR

Anti-Human IL-15 (9B5G1) MAb, Mouse IgG1 (Cat. No. IL5-M543) captured on CM5 chip via anti-mouse antibodies surface can bind Human IL-15, His Tag (Cat. No. IL5-H52H8) with an affinity constant of 2.76 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).

  • Background
    Interleukin 15 is also known as IL15, IL-15, and is a cytokine with structural similarity to IL-2. Like IL-2, IL-15 binds to and signals through the IL-2/IL-15 beta chain (CD122) and the common gamma chain (gamma-C, CD132). IL-15 is secreted by mononuclear phagocytes (and some other cells) following infection by virus(es). This cytokine induces cell proliferation of natural killer cells; cells of the innate immune system whose principal role is to kill virally infected cells. Interleukin 15 (IL-15) regulates T and natural killer (NK) cell activation and proliferation. Survival signals that maintain memory T cells in the absence of antigen are provided by IL-15. This cytokine is also implicated in NK cell development. In rodent lymphocytes, IL-15 prevents apoptosis by inducing an apoptosis inhibitor, BCL2L1/BCL-x(L). IL-15 has been shown to enhance the anti-tumor immunity of CD8+ T cells in pre-clinical models. A phase I clinical trial to evaluate the safety, dosing, and anti-tumor efficacy of IL-15 in patients with metastatic melanoma and renal cell carcinoma (kidney cancer) has begun to enroll patients at the National Institutes of Health.
  • Clinical and Translational Updates

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  • Number of Launched Drugs:0 Details
  • Number of Drugs in Clinical Trials:15 Details
  • Latest Research Phase:Phase 2 Clinical

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