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Human TRAC Protein, His Tag

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  • Synonym
    TRAC
  • Source
    Human TRAC, His Tag(TRC-H52H3) is expressed from human 293 cells (HEK293). It contains AA Ile 1 - Ser 115 (Accession # P01848-1).
    Predicted N-terminus: Ile 1
  • Molecular Characterization
    TRAC Structure

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 14.8 kDa. The protein migrates as 25-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
TRAC SDS-PAGE

Human TRAC, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
 TRAC ELISA

Immobilized Human TRAC, His Tag (Cat. No. TRC-H52H3) at 5 μg/mL (100 μL/well) can bind TRAC Antibody with a linear range of 0.2-5 ng/mL (Routinely tested).

  • Background
    The transmembrane protein, TCR, comprise of two disulphide-linked polypeptide chains: a α and β chain, a γ and δ chain. Each polypeptide chain consists of a variable and a constant region. TRAC is the constant region of T-cell receptor (TCR) alpha chain. TRAC is presented on the surface of T cell and recognized peptide-major histocompatibility (MH) (pMH) that are displayed by antigen presenting cells (APC). TRAC is participate in adaptive immune response and has been well-studied in T cell therapy.
  • Clinical and Translational Updates

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