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Your Position: Start > Antibody > Spike RBD > SPD-Y68

Anti-SARS-CoV-2 Spike RBD Broadly Antibody, Mouse IgG1 (2G7F5) (MALS verified)

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  • Source
    Anti-SARS-CoV-2 Spike RBD Broadly Antibody, Mouse IgG1 Mouse monoclonal antibody is produced from a hybridoma resulting from fusion of SP2/0 myeloma and B-lymphocytes obtained from a mouse immunized with Spike protein.
  • Clone

    2G7F5

  • Species

    Mouse

  • Isotype

    Mouse IgG1 | Mouse Kappa

  • Conjugate

    Unconjugated

  • Antibody Type

    Hybridoma Monoclonal

  • Reactivity

    Virus

  • Immunogen

    Recombinant SARS-CoV-2 Spike Trimer Protein (BA.4/Omicron) erived from human 293 cells.

  • Specificity

    This product can broadly reacts with SARS-CoV-2 Spike protein of WT and variant.

  • Application
    ApplicationRecommended Usage
    ELISA0.1-75 ng/mL
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Purification

    Protein A purified/ Protein G purified

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Spike RBD SDS-PAGE

Anti-SARS-CoV-2 Spike RBD Broadly Antibody, Mouse IgG1 (2G7F5) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS
Spike RBD MALS images

The purity of Anti-SARS-CoV-2 Spike RBD Broadly Antibody, Mouse IgG1 (2G7F5) (Cat. No. SPD-Y68) is more than 90% and the molecular weight of this protein is around 126-154 kDa verified by SEC-MALS.

Bioactivity-ELISA
 Spike RBD ELISA

Immobilized SARS-CoV-2 (COVID-19) S protein RBD (Cat. No. SPD-C52H3), Alpha (Cat. No. SPD-C52Hn), Beta (Cat. No. SPD-C52Hp), Gamma (Cat. No. SPD-C52Hr), Delta (Cat. No. SPD-C52Hh), B.1.1.529/Omicron (Cat. No. SPD-C522e), BA.2/Omicron (Cat. No. SPD-C522g), BA.2.12.1/Omicron (Cat. No. SPD-C522q), BA.4&BA.5/Omicron (Cat. No. SPD-C522r), BA.3/Omicron (Cat. No. SPD-C522i) and BA.2.75/Omicron (Cat. No. SPD-C522t) at 2μg/mL (100μL/well) can bind Anti-SARS-CoV-2 Spike RBD Broadly Antibody, Mouse IgG1 (2G7F5) (Cat. No. SPD-Y68) (QC tested).

  • Background
    It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
  • Clinical and Translational Updates

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