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Your Position: Start > Protein > NPR1 > NP1-H82E9

Biotinylated Human NPR1 / NPRA Protein, His,Avitag™ (MALS verified)

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  • Synonym
    Atrial natriuretic peptide receptor 1,NPR1,NPRA,GC-A,ANPRA,NPR-A
  • Source
    Biotinylated Human NPR1, His,Avitag (NP1-H82E9) is expressed from human 293 cells (HEK293). It contains AA Gly 33 - Glu 473 (Accession # P16066-1).
    Predicted N-terminus: Gly 33
  • Molecular Characterization
    Online(Gly 33 - Glu 473) P16066-1

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 52.5 kDa. The protein migrates as 63-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human NPR1, His,Avitag (Cat. No. NP1-H82E9) SDS-PAGE gel

Biotinylated Human NPR1, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Biotinylated Human NPR1, His,Avitag (Cat. No. ) MALS images

The purity of Biotinylated Human NPR1, His,Avitag (Cat. No. NP1-H82E9) is more than 85% and the molecular weight of this protein is around 65-80 kDa verified by SEC-MALS.

  • Background
    Receptor for the atrial natriuretic peptide NPPA/ANP and the brain natriuretic peptide NPPB/BNP which are potent vasoactive hormones playing a key role in cardiovascular homeostasis. Has guanylate cyclase activity upon binding of the ligand.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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