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Your Position: Start > Protein > CD43 > CD3-H52H9

Human CD43 / leukosialin Protein, His Tag (MALS & SPR verified)

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  • Synonym
    leukosialin, sialophorin, GPL115, Galactoglycoprotein, GALGP, Leukocyte sialoglycoprotein
  • Source
    Human CD43, His Tag (CD3-H52H9) is expressed from human 293 cells (HEK293). It contains AA Ser 20 - Arg 253 (Accession # P16150-1 ).
    Predicted N-terminus: Ser 20
  • Molecular Characterization
    Online(Ser 20 - Arg 253) P16150-1 

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 25.3 kDa. The protein migrates as 45-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human CD43, His Tag (Cat. No. CD3-H52H9) SDS-PAGE gel

Human CD43, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS
Human CD43, His Tag (Cat. No. ) MALS images

The purity of Human CD43, His Tag(Cat. No. CD3-H52H9) is more than 90% and the molecular weight of this protein is around 38-48 kDa verified by SEC-MALS.

Bioactivity-SPR
Human_FcRn_Heterodimer_Protein_SPR

Human Siglec-1, His Tag (Cat. No. SG1-H52H3) immobilized on CM5 Chip can bind Human CD43, His Tag (Cat. No. CD3-H52H9) with an affinity constant of 76.1 μM as determined in a SPR assay (Biacore 8K) (QC tested).

  • Background
    CD43 or leukosialin or sialophorin, is predominant cell surface sialoprotein of leukocytes which regulates multiple T-cell functions, including T-cell activation, proliferation, differentiation, trafficking and migration. In hematopathology, CD43 is often considered as a T-cell associated marker, because it is expressed by over 95% of thymocytes and peripheral blood T cells. CD43 positively regulates T-cell trafficking to lymph-nodes via its association with ERM proteins (EZR, RDX and MSN).
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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