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Your Position: Start > Protein > CCL7 > CC7-H52H6

Human CCL7 / MCP-3 Protein, His Tag

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  • Synonym
    C-C motif chemokine 7,Monocyte chemoattractant protein 3,Monocyte chemotactic protein 3,MCP-3,NC28,Small-inducible cytokine A7,CCL7,MCP3, SCYA6, SCYA7
  • Source
    Human CCL7, His Tag (CC7-H52H6) is expressed from human 293 cells (HEK293). It contains AA Gln 24 - Leu 99 (Accession # P80098-1).
    Predicted N-terminus: Gln 24
  • Molecular Characterization
    Online(Gln 24 - Leu 99) P80098-1

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 10.8 kDa. The protein migrates as 14 kDa and 18-20 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human CCL7, His Tag (Cat. No. CC7-H52H6) SDS-PAGE gel

Human CCL7, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

  • Background
    CCL7, also known as monocyte chemotactic protein-3 (MCP-3), is a CC (cysteine–cysteine) chemokine that is 11–18 kDa in size depending on the level of glycosylation. Its gene has been mapped to chromosome 17q11.2-q12 and is expressed in response to endogenous cytokines or exogenous bacterial products. This protein shares 60–71% homology with MCP-1 and MCP-2 chemokines and consequently binds similar receptors. Receptors CCR1, CCR2, CCR3, and CCR5 have been identified to bind CCL7 in both progenitor and immune cells.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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