This protein carries a polyhistidine tag at the N-terminus and a twin strep tag at the C-terminus
The protein has a calculated MW of 26.3 kDa. The protein migrates as 20 kDa and 40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>80% as determined by SDS-PAGE.
This product is not suitable for cell based experiments due to cytotoxicity of DDM.
DDM and CHS are INDISPENSABLE to keep membrane protein soluble and active, under no circumastance should you remove DDM and CHS.
DDM/CHS buffer (DC-11) is sold separately and not included in protein, and please contact us if you need the buffer.
If glycerol is not compatible to your application, remove glycerol just before immediate experiment, and NEVER store glycerol-free protein solution.
Supplied as 0.2 μm filtered solution in 50 mM HEPES, 150 mM NaCl, DDM, CHS, pH7.5 with glycerol as protectant.
Contact us for customized product form or formulation.
This product is supplied and shipped as sterile liquid solution with dry ice, please inquire the shipping cost.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human / Cynomolgus Claudin-18.2, His,Twin-Strep Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 80%.
Immobilized Human / Cynomolgus Claudin-18.2, His,Twin-Strep Tag (Cat. No. CL2-H5587) at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-Chimeric Claudin-18.2 Antibody, Human IgG1 with a linear range of 0.1-2 ng/mL (QC tested).
Immobilized Human / Cynomolgus Claudin-18.2, His,Twin-Strep Tag (Cat. No. CL2-H5587) and Human Claudin-18.1, His,Twin-Strep Tag ( Cat. No. CL1-H5588) at 5 μg/mL (100 μL/well) respectively, the Monoclonal Anti-Chimeric Claudin-18.2 Antibody, Human IgG1 can bind the Human / Cynomolgus Claudin-18.2, His,Twin-Strep Tag (Cat. No. CL2-H5587) with a linear range of 0.2-16 ng/mL, but can not bind the Human Claudin-18.1, His,Twin-Strep Tag (Cat. No. CL1-H5588) (Routinely tested).
Anti-Claudin-18.2 mAb captured on CM5 chip via anti-Human IgG (Fc) antibody can bind Human / Cynomolgus Claudin-18.2, His,Twin-Strep Tag (Cat. No. CL2-H5587) with an affinity constant of 6.72 nM as determined in a SPR assay (in presence of DDM and CHS) (Biacore T200) (Routinely tested).
Loaded Monoclonal Anti-Chimeric Claudin-18.2 Antibody, Human IgG1 on AHC Biosensor, can bind Human / Cynomolgus Claudin-18.2, His,Twin-Strep Tag (Cat. No. CL2-H5587) with an affinity constant of 3.03 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
Preis(EUR) : €1210.00
Preis(EUR) : €3540.00
Preis(EUR) : €8670.00
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