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Your Position: Start > Antibody > Spike RBD > SPD-M305

Anti-SARS-CoV-2 Spike RBD Antibody, Mouse IgG1 (AS113) (Omicron Specific)

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  • Source
    The mouse monoclonal antibody is produced from a hybridoma resulting from fusion of SP2/0 myeloma and B-lymphocytes obtained from a mouse immunized with Spike RBD. The antibody is specific against the Omicron (B.1.1.529/BA.1) variant of SARS-CoV-2, and has no binding with the spike RBD of the wild type virus and other viral lineages.
  • Clone

    AS113

  • Species

    Mouse

  • Isotype

    Mouse IgG1 | Mouse Kappa

  • Conjugate

    Unconjugated

  • Reactivity

    Virus

  • Immunogen

    Recombinant SARS-CoV-2 Spike RBD (B.1.1.529/Omicron) erived from human 293 cells.

  • Specificity

    This product is a specific antibody against Spike RBD of Omicron (B.1.1.529/BA.1) variant of SARS-CoV-2. Cross-reactivity with Spike protein RBD domain of other coronaviruses, including SARS-CoV, MERS-CoV, HCoV-229E, HCoV-NL63, HCoV-OC43 and HCoV-HKU1, has not been tested.

  • Application
    ApplicationRecommended Usage
    ELISA0.4-200 ng/mL
  • Purity

    >95% as determined by SDS-PAGE.

  • Purification

    Protein A purified / Protein G purified

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Spike RBD SDS-PAGE

Anti-SARS-CoV-2 Spike RBD Antibody, Mouse IgG1 (AS113) (Omicron Specific) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

Bioactivity-ELISA
 Spike RBD ELISA

Immobilized SARS-CoV-2 Spike RBD, His Tag (B.1.1.529/Omicron) (Cat. No. SPD-C522e) can bind Anti-SARS-CoV-2 Spike RBD Antibody, Mouse IgG1 (AS113) (Omicron Specific) (Cat. No. SPD-M305) with a linear range of 0.4-12.5 ng/mL (QC tested). The antibody does not bind Spike RBD of WT (Cat. No. SPD-C52H1), Alpha (Cat. No. SPD-C52Hn), Beta (Cat. No. SPD-C52Hp), Delta (Cat. No. SPD-C52Hh) and Gamma (Cat. No. SPD-C52Hr).

Bioactivity-BLI
 Spike RBD BLI

Loaded Anti-SARS-CoV-2 Spike RBD Antibody, Mouse IgG1 (AS113) (Omicron Specific) (Cat. No. SPD-M305) on AMC Biosensor, can bind SARS-CoV-2 Spike RBD, His Tag (B.1.1.529/Omicron) (Cat. No. SPD-C522e) with an affinity constant of 9.07 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

  • Background
    It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
  • Clinical and Translational Updates

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