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Your Position: Start > Protein > IL-12B > NK2-M82E5

Biotinylated Mouse IL-12B / NKSF2 / p40 Protein, His,Avitag™ (MALS verified)

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  • Synonym
    IL12B,CLMF,CLMF2,NKSF,NKSF2,p40
  • Source
    Biotinylated Mouse IL-12B, His,Avitag(NK2-M82E5) is expressed from human 293 cells (HEK293). It contains AA Met 23 - Ser 335 (Accession # P43432-1).
    Predicted N-terminus: Met 23
  • Molecular Characterization
    IL-12B Structure

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™)

    The protein has a calculated MW of 39.4 kDa. The protein migrates as 45 kDa,50 kDa and 55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
IL-12B SDS-PAGE

Biotinylated Mouse IL-12B, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS
IL-12B MALS images

The purity of Biotinylated Mouse IL-12B, His,Avitag (Cat. No. NK2-M82E5) is more than 95% and the molecular weight of this protein is around 36-54 kDa verified by SEC-MALS.

Bioactivity-ELISA
 IL-12B ELISA

Immobilized Biotinylated Mouse IL-12B, His,Avitag (Cat. No. NK2-M82E5) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human IL-12 R beta 1, Fc Tag (Cat. No. ILB-H5255) with a linear range of 0.02-0.156 μg/mL (QC tested).

  • Background
    Interleukin-12 (IL12) is also known as natural killer cell stimulatory factor (NKSF), cytotoxic lymphocyte maturation factor (CLMF) , is a heterodimeric cytokine encoded by two separate genes, IL-12A (p35) and IL-12B (p40). IL12 is naturally produced by dendritic cells, macrophages and human B-lymphoblastoid cells (NC-37) in response to antigenic stimulation. IL-12 is involved in the differentiation of naive T cells into Th0 cells and plays an important role in the activities of natural killer cells and T lymphocytes.IL-12 also has anti-angiogenic activity, which means it can block the formation of new blood vessels.
    Interleukin-12 subunit beta (IL12B) also known as NKSF2, CLMF2 and P40. Interleukin-12 subunit beta has been shown to interact with IL23. A large excess of monomeric IL12B is also secreted by the cells producing IL12, and exhibits no demonstrable biological activity. Overexpression of IL12B gene has been shown to be associated with the pathogenesis of multiple sclerosis. In addition, studies have revealed that the promoter polymorphism of this gene is implicated in the severity of atopic and non-atopic asthma in children.
  • Clinical and Translational Updates

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  • Latest Research Phase:Approved

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