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Your Position: Start > Protein > SIRP gamma > SIG-H82E3

Biotinylated Human SIRP gamma / CD172g Protein, His,Avitag™ (MALS verified)

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  • Synonym
    SIRPG,CD172g,SIRPB2,SIRP-gamma,SIRP-b2,SIRP-beta-2,Cripto-1
  • Source
    Biotinylated Human SIRP gamma, His,Avitag(SIG-H82E3) is expressed from human 293 cells (HEK293). It contains AA Glu 29 - Ser 364 (Accession # AAH64532.1).
    Predicted N-terminus: Glu 29
  • Molecular Characterization
    SIRP gamma Structure

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 40.7 kDa. The protein migrates as 48-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
SIRP gamma SDS-PAGE

Biotinylated Human SIRP gamma, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
SIRP gamma MALS images

The purity of Biotinylated Human SIRP gamma, His,Avitag (Cat. No. SIG-H82E3) is more than 90% and the molecular weight of this protein is around 45-57 kDa verified by SEC-MALS.

Bioactivity-ELISA
 SIRP gamma ELISA

Immobilized Biotinylated Human SIRP gamma, His,Avitag (Cat. No. SIG-H82E3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human CD47, Mouse IgG2a Fc Tag (Cat. No. CD7-H52A5) with a linear range of 4-31 ng/mL (QC tested).

  • Background
    Signal-regulatory protein gamma (SIRPG or SIRP-gamma) is also known as CD172 antigen-like family member B (CD172g), Signal-regulatory protein beta-2 (SIRPB2), is a single-pass type I membrane protein. SIRPG is expressed on CD4+ T-cells, CD8+ T-cells, CD56-bright natural killer (NK) cells, CD20+ cells, and all activated NK cells. SIRPG interacts with CD47. On binding with CD47, CD172g mediates cell-cell adhesion.
  • Clinical and Translational Updates

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