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Your Position: Start > Antibody > Spike RBD > SPD-BM227

Biotinylated Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 (AM130) (MALS verified)

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  • Source
    The Mouse monoclonal antibody is produced from a hybridoma resulting from fusion of SP2/0 myeloma and B-lymphocytes obtained from a mouse immunized with Spike RBD F(ab')2.
  • Clone

    AM130

  • Isotype

    Human IgG1 | Human Kappa

  • Conjugate

    Biotin

  • Reactivity

    Virus

  • Specificity

    This product can recognize SARS-CoV-2 and SARS-CoV Spike Protein RBD domain. No cross-reactivity is detected with Spike Protein RBD domain of other coronaviruses, including MERS-CoV, HCoV-229E, HCoV-NL63, HCoV-OC43 and HCoV-HKU1.

  • Application
    ApplicationRecommended Usage
    ELISA1.6-1000 ng/mL
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Purification

    Protein A purified/ Protein G purified

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.3 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Spike RBD SDS-PAGE

Biotinylated Anti-SARS-CoV-2 Spike RBD Antibody, Human IgG1 (AM130) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Spike RBD MALS images

The purity of Biotinylated Anti-SARS-CoV-2 Spike RBD Antibody, Human IgG1 (AM130) (Cat. No. SPD-BM227) is more than 90% and the molecular weight of this protein is around 145-155 kDa verified by SEC-MALS.

Bioactivity-ELISA
 Spike RBD ELISA

Immobilized SARS-CoV-2 S protein RBD, His Tag(Cat. No. SPD-C52H3) at 2 μg/mL (100 μL/well) can bind Biotinylated Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 (AM130) (Cat. No. SPD-BM227) with a linear range of 0.195-25ng/mL (QC tested)

  • Background
    It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
  • Clinical and Translational Updates

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