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This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 8.3 kDa. The protein migrates as 10-11 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human EGF Protein, His Tag, premium grade on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
EGF (Cat. No. EGF-H52H3) maintains intestinal organoid growth well through multiple passages and long-term culture, comparable to competing companies. The organoids showed good budding morphology.
Human EGF (Cat. No. EGF-H52H3), Noggin (Cat. No. NON-H5257), R-spondin1 (Cat. No. RS6-H4220), FGF7 (Cat. No. FG7-H52H5), FGF10 (Cat. No. FG0-H5145), HGF (Cat. No. HGF-H52H3) actively support liver ductal organoid growth.
Human EGF Protein, His Tag, premium grade (Cat. No. EGF-H52H3) stimulates proliferation of 293F-STAT3-EGFR-24 cells. The specific activity of Human EGF Protein, His Tag, premium grade is＞8.00 X 10^5 IU/mg, which is calibrated against human growth factor EGF WHO International Standard (NIBSC code: 91/530) (QC tested).
Immobilized Human EGF Protein, His Tag, premium grade (Cat. No. EGF-H52H3) at 2 μg/mL (100 μL/well) can bind Human EGF R, Fc Tag (Cat. No. EGR-H5252) with a linear range of 0.2-5 ng/mL (QC tested).
Price(EUR) : €60.00
Price(EUR) : €160.00
Price(EUR) : €240.00
Price(EUR) : €1600.00
Price(EUR) : €250.00
Price(EUR) : €1700.00
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