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FITC-Labeled Human CD7 Protein, Llama IgG2b Fc Tag

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  • Synonym
    CD7,GP40,TP41,LEU-9,Tp40
  • Source
    FITC-Labeled Human CD7, Llama IgG2b Fc Tag (CD7-HF257) is expressed from human 293 cells (HEK293). It contains AA Ala 26 - Pro 180 (Accession # P09564-1). It is the FITC labeled form of Human CD7, Llama IgG2b Fc Tag, low endotoxin (CD7-H5258).
    Predicted N-terminus: Glu
  • Molecular Characterization
    CD7 Structure

    This protein carries a llama IgG2b Fc tag at the N-terminus.

    The protein has a calculated MW of 44.3 kDa. The protein migrates as 55-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Conjugate

    FITC

    Excitation source: 488 nm spectral line, argon-ion laser

    Excitation Wavelength: 488 nm

    Emission Wavelength: 535 nm

  • Labeling
    The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with FITC using standard chemical labeling method. The residual FITC is removed by molecular sieve treatment during purification process.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
CD7 SDS-PAGE

FITC-Labeled Human CD7, Llama IgG2b Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
 CD7 ELISA

Immobilized Anti-CD7 antibody, Mouse IgG1 at 1 μg/mL (100 μL/well) can bind FITC-Labeled Human CD7, Llama IgG2b Fc Tag (Cat. No. CD7-HF257) with a linear range of 0.039-0.625 ug/mL (QC tested).

  • Background
    T-cell antigen CD7 (CD7) is also known as GP40, LEU-9, TP41 and Tp40. CD7 is a protein that in humans is encoded by the CD7 gene, this gene encodes a transmembrane protein which is a member of the immunoglobulin superfamily. CD7 has been shown to interact with PIK3R1. This protein is found on thymocytes and mature T cells. It plays an essential role in T-cell interactions and also in T-cell/B-cell interaction during early lymphoid development.
  • Clinical and Translational Updates

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  • Number of Drugs in Clinical Trials:24 Details
  • Latest Research Phase:Phase 2 Clinical

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