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Your Position: Start > Protein > CD7 > CD7-HF2H6

FITC-Labeled Human CD7 Protein, His Tag

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  • Synonym
    CD7,GP40,TP41,LEU-9,Tp40
  • Source
    FITC-Labeled Human CD7, His Tag (CD7-HF2H6) is expressed from human 293 cells (HEK293). It contains AA Ala 26 - Pro 180 (Accession # P09564-1). It is the FITC labeled form of Human CD7, His Tag (CD7-H52H7).
    Predicted N-terminus: Ala 26
  • Molecular Characterization
    CD7 Structure

    This protein carries a polyhistidine tag at the C-terminus

    The protein has a calculated MW of 18.3 kDa. The protein migrates as 25-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Conjugate

    FITC

    Excitation source: 488 nm spectral line, argon-ion laser

    Excitation Wavelength: 488 nm

    Emission Wavelength: 535 nm

  • Labeling
    The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with FITC using standard chemical labeling method. The residual FITC is removed by molecular sieve treatment during purification process.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
CD7 SDS-PAGE

FITC-Labeled Human CD7, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-FACS
 CD7 FACS

2e5 of Anti-CD7 CAR-293 cells were stained with 100 μL of 10 μg/mL of FITC-Labeled Human CD7, His Tag (Cat. No. CD7-HF2H6) and negative control protein respectively, FITC signals was used to evaluate the binding activity (Routinely tested).

  • Background
    T-cell antigen CD7 (CD7) is also known as GP40, LEU-9, TP41 and Tp40. CD7 is a protein that in humans is encoded by the CD7 gene, this gene encodes a transmembrane protein which is a member of the immunoglobulin superfamily. CD7 has been shown to interact with PIK3R1. This protein is found on thymocytes and mature T cells. It plays an essential role in T-cell interactions and also in T-cell/B-cell interaction during early lymphoid development.
  • Clinical and Translational Updates

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