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Your Position: Start > Protein > CD40 Ligand > CDL-H52Db

Human CD40 Ligand / TNFSF5 Protein, His,Flag Tag, active trimer (MALS verified)

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  • Synonym
    CD40LG,CD154,CD40L,HIGM1,IGM,IMD3,T-BAM,TNFSF5,TRAP,gp39
  • Source
    Human CD40 Ligand, His,Flag Tag (CDL-H52Db) is expressed from human 293 cells (HEK293). It contains AA Gly 116 - Leu 261 (Accession # P29965-1 ) trimer Design.
    Predicted N-terminus: His
  • Molecular Characterization

    This protein carries a polyhistidine tag at the N-terminus, followed by a flag tag.

    The protein has a calculated MW of 49.9 kDa. The protein migrates as 45-55 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE).

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
CD40 Ligand SDS-PAGE

Human CD40 Ligand, His,Flag Tag on SDS-PAGE under non-reducing (NR) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS
CD40 Ligand MALS images

The purity of Human CD40 Ligand, His,Flag Tag (Cat. No. CDL-H52Db) is more than 85% and the molecular weight of this protein is around 51-69 kDa verified by SEC-MALS.

Bioactivity-ELISA
 CD40 Ligand ELISA

Immobilized Human CD40 Ligand, His,Flag Tag (Cat. No. CDL-H52Db) at 5 μg/mL (100 μL/well) can bind Human CD40 Protein, Mouse IgG2a Fc Tag (Cat. No. CD0-H525a) with a linear range of 0.01-0.3 μg/mL (QC tested).

 CD40 Ligand ELISA

Immobilized Biotinylated Human CD40, Fc,Avitag (Cat. No. TN5-H82F9) at 5 μg/mL (100 μL/well) on Streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate, can bind Human CD40 Ligand, His,Flag Tag (Cat. No. CDL-H52Db) with a linear range of 0.039-1.25 μg/mL (Routinely tested).

Bioactivity-BLI
 CD40 Ligand BLI

Loaded Human CD40, Fc Tag (Cat. No. CD0-H5253) on Protein A Biosensor, can bind Human CD40 Ligand, His,Flag Tag (Cat. No. CDL-H52Db) with an affinity constant of 1.58 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Bioactivity-Bioactivity CELL BASE
 CD40 Ligand CELL

Human CD40 Ligand, His,Flag Tag (Cat. No. CDL-H52Db) stimulates secretion of IL-6 by human B cells. The ED50 for this effect is 18.76-19.45 ng/mL in the presence of 5 ng/mL of Recombinant Human IL‑4 (Routinely tested).

 CD40 Ligand CELL

Neutralization assay shows that the cytokine secretion effect of Human CD40 Ligand, His,Flag Tag (Cat. No. CDL-H52Db) was inhibited by increasing concentration of anti-CD40L mAb. The concentration of CD40L used is 100 ng/mL. The EC50 is 0.215 μg/mL (Routinely tested).

  • Background
    CD40 ligand is also known as CD40L, CD154, TNFSF5 and T-cell antigen Gp39, is a single-pass type I I membrane protein which belongs to the TNF superfamily of molecules. CD40 ligand is expressed predominantly on activated CD4+ T lymphocytes, and also found in other types of cells, including platelets, mast cells, macrophages, basophils, NK cells, B lymphocytes, as well as non-haematopoietic cells (smooth muscle cells, endothelial cells, and epithelial cells). Although all monomeric, dimeric and trimeric forms of soluble CD40 ligand can bind to CD40, the trimeric form of soluble CD40 ligand has the most potent biological activity through oligomerization of cell surface CD40, a common feature of TNF receptor family members.
    CD40 ligand binds to CD40 on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. In general, CD40 ligand plays the role of a costimulatory molecule and induces activation in APC in association with T cell receptor stimulation by MHC molecules on the APC. In total CD40 ligand has three binding partners: CD40, α5β1 integrin and αIIbβ3. CD40 ligand regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper IgM syndrome.
  • Clinical and Translational Updates
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(EUR) : €350.00

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