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Biotinylated Cynomolgus / Rhesus macaque FcRn Heterodimer Protein, His,Avitag, produced by co-expression of FCGRT and B2M, has a calculated MW of 34.1 kDa (FCGRT) and 11.6 kDa (B2M). Subunit FCGRT is fused with a polyhistidine tag at the C-terminus , followed by an Avi tag (Avitag™) and subunit Beta-2 microglobulin (B2M) contains no tag at the C-terminus. The reducing (R) protein migrates as 35-40 kDa (FCGRT) and 13 kDa (B2M) respectively due to glycosylation.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Biotinylated Cynomolgus / Rhesus macaque FcRn Heterodimer Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Biotinylated Cynomolgus / Rhesus macaque FcRn Heterodimer Protein, His,Avitag (Cat. No. FCM-C82W3) is more than 90% and the molecular weight of this protein is around 45-55 kDa verified by SEC-MALS.
Biotinylated Cynomolgus / Rhesus macaque FcRn Heterodimer Protein, His,Avitag (Cat. No. FCM-C82W3) captured on Biotin CAP-Series S Sensor Chip can bind Herceptin® with an affinity constant of 0.119 μM as determined in a SPR assay (Biacore T200) (QC tested).
Loaded Biotinylated Cynomolgus / Rhesus macaque FcRn Heterodimer Protein, His,Avitag (Cat. No. FCM-C82W3) on SA Biosensor, can bind Cynomolgus Serum Albumin, His Tag (Cat. No. CSA-C52H4) with an affinity constant of 0.617 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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