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Biotinylated Cynomolgus CD3E&CD3D Heterodimer Protein, His,Avitag&Tag Free is produced by co-expression of CD3E and CD3D, has a calculated MW of 17.8 kDa (CD3E) and 13.5 kDa (CD3D). Subunit CD3E is fused with a polyhistidine tag and followed by an Avi tag (Avitag™) and subunit CD3D contains no tag at the C-terminus. The reducing (R) heterodimer protein migrates as 21-23 kDa (CD3E) and 19-21 kDa (CD3D) when calibrated against Star Ribbon Pre-stained Protein Marker due to glyosylation.
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Biotinylated Cynomolgus CD3E&CD3D Heterodimer Protein, His,Avitag&Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
The purity of Biotinylated Cynomolgus CD3E&CD3D Heterodimer Protein, His,Avitag&Tag Free (Cat. No. CDD-C82W6) is more than 90% and the molecular weight of this protein is around 38-50 kDa verified by SEC-MALS.
Immobilized Bispecific T cell Engager (CD3 X BCMA) at 1 μg/mL (100 μL/well) can bind Biotinylated Cynomolgus CD3E&CD3D Heterodimer Protein, His,Avitag&Tag Free (Cat. No. CDD-C82W6) with a linear range of 0.5-3.9 ng/mL (Intact assay, QC tested).
Bispecific T-cell Engager (CD3 X BCMA) immobilized on CM5 Chip can bind Biotinylated Cynomolgus CD3E&CD3D Heterodimer Protein, His,Avitag&Tag Free (Cat. No. CDD-C82W6) with an affinity constant of 53.5 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Loaded Biotinylated Cynomolgus CD3E&CD3D Heterodimer Protein, His,Avitag&Tag Free (Cat. No. CDD-C82W6) on SA Biosensor, can bind Human CD ×BCMA scFV with an affinity constant of 1.84 nM as determined in BLI assay (ForteBio Octet Red9e) (Routinely tested).
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