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Human CD19 (20-291) Protein, Mouse IgG2a Fc Tag (MALS verified)

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  • Synonym
    CD19,B4,CVID3,MGC12802
  • Source
    Human CD19 (20-291), Mouse IgG2a Fc Tag(CD9-H5258) is expressed from human 293 cells (HEK293). It contains AA Pro 20 - Lys 291 (Accession # P15391-1).
    Predicted N-terminus: Pro 20
  • Molecular Characterization
    CD19 Structure

    This protein carries a mouse IgG2a Fc tag at the C-terminus.

    The protein has a calculated MW of 57.0 kDa. The protein migrates as 75 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 0.1 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
CD19 SDS-PAGE

Human CD19 (20-291), Mouse IgG2a Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS
CD19 MALS images

The purity of Human CD19 (20-291), Mouse IgG2a Fc Tag (Cat. No. CD9-H5258) is more than 90% and the molecular weight of this protein is around 130-160 kDa verified by SEC-MALS.

Bioactivity-ELISA
 CD19 ELISA

Immobilized Human CD19 (20-291), Mouse IgG2a Fc Tag (Cat. No. CD9-H5258) at 5 μg/mL (100 μL/well) can bind Mouse / Human chimeric FMC63 MAb (human IgG1) with a linear range of 0.5-8 ng/mL (QC tested).

Bioactivity-BLI
 CD19 BLI

Loaded Mouse / Human chimeric FMC63 MAb (human IgG1) on AHC Biosensor, can bind Human CD19 (20-291), Mouse IgG2a Fc Tag (Cat. No. CD9-H5258) with an affinity constant of 0.487 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

  • Background
    B-lymphocyte antigen CD19 is also known as CD19 (Cluster of Differentiation 19), is a single-pass type I  membrane protein which contains two Ig-like C2-type (immunoglobulin-like) domains. CD19 is expressed on follicular dendritic cells and B cells. In fact, it is present on B cells from earliest recognizable B-lineage cells during development to B-cell blasts but is lost on maturation to plasma cells. It primarily acts as a B cell co-receptor in conjunction with CD21 and CD81. Upon activation, the cytoplasmic tail of CD19 becomes phosphorylated, which leads to binding by Src-family kinases and recruitment of PI-3 kinase. As on T cells, several surface molecules form the antigen receptor and form a complex on B lymphocytes. The (almost) B cell-specific CD19 phosphoglycoprotein is one of these molecules. The others are CD21 and CD81. These surface immunoglobulin (sIg)-associated molecules facilitate signal transduction. On living B cells, anti-immunoglobulin antibody mimicking exogenous antigen causes CD19 to bind to sIg and internalize with it. The reverse process has not been demonstrated, suggesting that formation of this receptor complex is antigen-induced. This molecular association has been confirmed by chemical studies. Mutations in CD19 are associated with severe immunodeficiency syndromes characterized by diminished antibody production. CD19 has been shown to interact with: CD81, CD82, Complement receptor 2, and VAV2.
  • Clinical and Translational Updates

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